Fertilizability of in vitro matured oocytes from golden hamsters.
Fertilizability of in vitro matured oocytes from golden hamsters.
复制标题
金仓鼠体外成熟卵母细胞的受精能力。
DOI：
10.1002/jez.1402260320
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Bavister,BD
中科院分区：
文献类型：
--
作者：
Leibfried,ML;Bavister,BD
研究了体外成熟的仓鼠卵母细胞的受精率，以及影响培养核成熟的两个潜在因素。没有积云的卵母细胞核成熟所必需的四种氨基酸（异亮氨酸、蛋氨酸、苯丙氨酸和谷氨酰胺）（Gwatkin和Haidri， 1974）如果卵母细胞在发情前（第4天）与完整的积云一起培养，则不需要。尽管在发情周期第3天恢复的卵泡卵母细胞体外成熟的频率略低于第4天恢复的卵泡卵母细胞（分别为76%和95%），但它们仍然具有相当大的自发成熟频率。第3天恢复的卵泡卵母细胞与体外成熟的卵母细胞孵育6小时后，受精率与输卵管卵母细胞相当（分别为80%和82%）。精子/卵子孵育仅4小时后，卵泡卵母细胞的穿透率较低（37.4%），表明体外成熟的卵泡卵母细胞延迟了精子的穿透。4小时的孵育时间足以使80%或更多的卵母细胞渗透。98%穿透的卵泡卵母细胞正常受精，只有2%穿透的卵泡卵母细胞正常。大多数（85%）卵泡卵母细胞与输卵管卵母细胞不同，在精卵孵育6小时后不能引起精核去浓缩。使用高度定义的系统进行仓鼠配子的体外受精提供了严格的证据，证明分离的卵丘-卵母细胞复合物在体外不经历完全成熟。
The fertilizability of hamster oocytes matured in vitro was examined along with two factors potentially affecting nuclear maturation in culture. The four amino acids (isoleucine, methionine, phenylalanine, and glutamine) necessary for nuclear maturation of cumulus‐free oocytes (Gwatkin and Haidri, '74) were not required if oocytes recovered on the morning of proestrus (day 4) were cultured with intact cumuli. Although follicular oocytes recovered on day 3 of the estrous cycle (late diestrus) had somewhat lower frequencies of maturation in vitro compared to those recovered on day 4 (76 vs. 95%, respectively), they still had a substantial frequency of spontaneous maturation.Follicular oocytes recovered on day 3 and matured in vitro were fertilized at frequencies equivalent to oviducal oocytes (80 vs. 82%, respectively) when incubation of oocytes with precapacitated sperm was continued for 6 h. Penetration of follicular oocytes was lower (37.4%) after only 4 h of sperm/egg incubation, indicating a delay in sperm penetration with follicular oocytes matured in vitro. Incubation for 4 h is sufficient time for penetration of 80% or more of oviducal oocytes. While 98% of penetrated oviducal oocytes were fertilized normally, only 2% of penetrated follicular oocytes were normal. The majority (85%) of follicular oocytes, unlike oviducal oocytes, were unable to cause decondensation of sperm nuclei after 6 h of sperm/egg incubation. Use of a highly defined system for in vitro fertilization of hamster gametes has provided rigorous proof that isolated cumulus‐oocytes complexes do not undergo complete maturation in vitro.