Vasopressin-stimulated phosphoinositide hydrolysis in cultured rat inner medullary collecting duct cells is mediated by the oxytocin receptor.
Vasopressin-stimulated phosphoinositide hydrolysis in cultured rat inner medullary collecting duct cells is mediated by the oxytocin receptor.
复制标题
培养的大鼠内髓集合管细胞中加压素刺激的磷酸肌醇水解是由催产素受体介导的。
DOI：
10.1172/jci115243
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Teitelbaum,I
中科院分区：
文献类型：
--
作者：
Teitelbaum,I
本研究旨在鉴定在培养的大鼠内髓集合管（RIMCT）细胞中介导AVP刺激的磷酸肌醇（PI）水解的受体。选择性V1受体激动剂[Ho 1，Phe 2，Orn 8] VT在10（-13）-10（-7）M范围内对三磷酸肌醇（IP 3）的产生没有影响，而选择性V2受体激动剂VDAVP以剂量依赖性方式刺激IP 3的产生。催产素也以剂量依赖的方式刺激IP 3的产生。AVP刺激的磷脂酶C活性不受V1受体拮抗剂d（CH 2）5 Tyr（Me）AVP（10（-7）M）的抑制，但可被V2受体拮抗剂d（CH 2）5DTyr（Et）VAVP（10（-7）M）消除。类似地，对催产素的反应被V2受体拮抗剂消除。选择性催产素受体激动剂[Thr 4，Gly 7]催产素不刺激RIMCT细胞中的cAMP产生，但促进PI水解。选择性催产素受体拮抗剂desGlyNH 2d（CH 2）5[Tyr（Me）-Thr 4]OVT（10（-7）M）不抑制AVP刺激的cAMP产生，但消除响应AVP或V2受体激动剂VDAVP的IP 3产生。这些研究表明，AVP或V2受体激动剂通过占据催产素受体刺激培养的RIMCT细胞中的PI水解。
Studies were performed to identify the receptor that mediates AVP-stimulated phosphoinositide (PI) hydrolysis in cultured rat inner medullary collecting tubule (RIMCT) cells. While the selective V1 receptor agonist [Ho1, Phe2, Orn8] VT has no effect on inositol trisphosphate (IP3) production over the range of 10(-13)-10(-7) M, the selective V2 receptor agonist VDAVP stimulates IP3 production in dose-dependent fashion. Oxytocin stimulates IP3 production in dose-dependent fashion as well. AVP-stimulated phospholipase C activity is not inhibited by the V1 receptor antagonist d(CH2)5Tyr(Me)AVP(10(-7) M) but is eliminated by the V2 receptor antagonist d(CH2)5DTyr(Et)VAVP (10(-7) M). Similarly, the response to oxytocin is eliminated by the V2 receptor antagonist. The selective oxytocin receptor agonist [Thr4, Gly7] oxytocin does not stimulate cAMP production in RIMCT cells but does promote PI hydrolysis. The selective oxytocin receptor antagonist desGlyNH2d(CH2)5[Tyr(Me)-Thr4]OVT (10(-7) M) does not inhibit AVP-stimulated cAMP production but eliminates IP3 production in response to AVP or the V2 receptor agonist VDAVP. These studies demonstrate that AVP or a V2 receptor agonist stimulate PI hydrolysis in cultured RIMCT cells via occupancy of the oxytocin receptor.