Temporal phases of activity-dependent plasticity and memory are mediated by compartmentalized routing of MAPK signaling in aplysia sensory neurons.
Temporal phases of activity-dependent plasticity and memory are mediated by compartmentalized routing of MAPK signaling in aplysia sensory neurons.
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DOI：
10.1016/j.neuron.2008.10.049
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发表时间：
2009-01-15
期刊：
影响因子：
16.2
通讯作者：
Carew TJ
中科院分区：
文献类型：
--
作者：
Shobe JL;Zhao Y;Stough S;Ye X;Hsuan V;Martin KC;Carew TJ
在海兔，一种用于敏化的活动依赖的中间记忆形式(AD-ITM)是由一次尾部电击诱导的，它通过尾部感觉神经元(SN)的动作电位放电和中枢神经系统中5-羟色胺的释放相互作用而引起尾部感觉神经元(SN)的可塑性变化(AD-ITF)。活性依赖的长期促进(AD-LTF，持续24小时)需要依赖于蛋白质合成的持续丝裂原激活蛋白激酶(MAPK)激活和转位到黑质核团。我们现在证明，早期时间阶段(AD-ITM和AD-ITF)的诱导是翻译和转录无关的，需要激活一个分隔不同的新的信号级联，将第二信使、MAPK和PKC连接到Tail SNS内的一条统一通路中。由于AD-ITM和AD-LTM都需要MAPK活性，这些共同的发现表明，突触前SNs在活动依赖的长期记忆的诱导过程中，将分子信息流传递到不同的亚细胞室。
An activity-dependent form of intermediate memory (AD-ITM) for sensitization is induced in Aplysia by a single tail shock that gives rise to plastic changes (AD-ITF) in tail sensory neurons (SNs) via the interaction of action potential firing in the SN coupled with the release of serotonin in the CNS. Activity-dependent long-term facilitation (AD-LTF, lasting >24hr) requires protein synthesis dependent persistent mitogen-activated protein kinase (MAPK) activation and translocation to the SN nucleus. We now show that the induction of the earlier temporal phase (AD-ITM and AD-ITF), which is translation and transcription independent, requires the activation of a compartmentally distinct novel signaling cascade that links second messengers, MAPK and PKC into a unified pathway within tail SNs. Since both AD-ITM and AD-LTM require MAPK activity, these collective findings suggest that presynaptic SNs route the flow of molecular information to distinct subcellular compartments during the induction of activity-dependent long-lasting memories.