Increasing expression of H- or L-ferritin protects cortical astrocytes from hemin toxicity.
Increasing expression of H- or L-ferritin protects cortical astrocytes from hemin toxicity.
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DOI：
10.1080/10715760902942808
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发表时间：
2009-06
影响因子：
3.3
通讯作者：
Regan RF
中科院分区：
文献类型：
--
作者：
Li Z;Chen-Roetling J;Regan RF
铁毒性可能导致脑内血肿周围细胞的氧化损伤。细胞通过将铁螯合在铁蛋白中来解毒铁，铁蛋白是由H和L亚基构成的24聚体杂聚物。H和L-铁蛋白的相对抗氧化功效尚未定义，并且在本研究中使用已建立的氯化血红素毒性模型进行了测试。与之前的观察结果一致，通过LDH和MTT测定，用30 μM氯化血红素处理的培养物在6小时内持续损失约一半的细胞，蛋白质羰基增加14倍。在氯化血红素治疗之前通过腺病毒基因转移增加铁蛋白的表达具有类似的保护作用。淬灭钙黄绿素荧光，标记的不稳定的铁池，在氯化血红素处理的文化也同样减少了任一亚基。这些结果表明，无论是H或L铁蛋白的过度表达保护星形胶质细胞从氯化血红素，并可能是有益的CNS出血后。
Iron toxicity may contribute to oxidative injury in cells surrounding an intracerebral hematoma. Cells detoxify iron by sequestering it in ferritin, a 24-mer heteropolymer constructed of H and L subunits. The relative antioxidant efficacy of H and L-ferritin has not been defined, and was tested in this study using an established model of hemin toxicity. Consistent with prior observations, cultures treated with 30 μM hemin sustained loss of approximately half of cells by six hours, as measured by LDH and MTT assays, and a 14-fold increase in protein carbonyls. Increasing expression of either ferritin by adenoviral gene transfer prior to hemin treatment had a similar protective effect. Quenching of calcein fluorescence, a marker of the labile iron pool, in hemin-treated cultures was also equally reduced by either subunit. These results suggest that over-expression of either H or L ferritin protects astrocytes from hemin, and may be beneficial after CNS hemorrhage.
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影响因子：
4.8
作者：
Ferreira, C;Bucchini, D;Beaumont, C
通讯作者：
Beaumont, C
影响因子：
20.3
作者：
Cozzi, A;Corsi, B;Arosio, P
通讯作者：
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
1.7
作者：
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通讯作者：
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