Prothymosin alpha gene in humans: organization of its promoter region and localization to chromosome 2.
Prothymosin alpha gene in humans: organization of its promoter region and localization to chromosome 2.
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人类中的胸腺素α基因：其启动子区域的组织和定位到2号染色体。
DOI：
10.1007/bf00202480
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发表时间：
1993
期刊：
影响因子：
5.3
通讯作者：
Horecker,BL
中科院分区：
文献类型：
--
作者：
Szabo,P;Panneerselvam,C;Clinton,M;Frangou-Lazaridis,M;Weksler,D;Whittington,E;Macera,MJ;Grzeschik,KH;Selvakumar,A;Horecker,BL
分离了编码前胸腺肽 α（基因符号：PTMA）（一种与细胞增殖相关的核靶向蛋白）的基因组克隆，并对 5' 调节区进行了亚克隆和测序。由于先前报道的几个 cDNA 克隆和胸腺肽原 α 基因组克隆之间的差异，我们确定了第一个外显子和第一个外显子 5' 的 1.7-kb 区域的序列。这里报告的基因组克隆的序列与已发表的 cDNA 序列相对应，表明先前提到的差异可能是由于该区域的遗传多态性造成的。此外，我们的序列数据将已知的 5'-上游序列额外延伸了 1.5 kb，从而能够识别大量潜在的顺式作用调控位点。这种 5' 侧翼克隆探针使我们能够将原胸腺素基因定位于人类 2 号染色体上。
A genomic clone encoding prothymosin α (gene symbol: PTMA), a nuclear-targeted protein associated with cell proliferation, was isolated and the 5′-regulatory region subcloned and sequenced. Because of previously reported discrepancies between several cDNA clones and a genomic clone for prothymosin α, we determined the sequence of the first exon and of a 1.7-kb region 5′ to the first exon. The sequence of the genomic clone reported here corresponds to the published cDNA sequences, suggesting that the previously noted discrepancies may be due to genetic polymorphism in this region. In addition, our sequence data extend the known 5′-upstream sequence by an additional 1.5 kb allowing the identification of numerous, potentialcis-acting regulatory sites. This 5′-flanking cloned probe permitted us to localize the protothymosin gene to chromosome 2 in humans.