Polystyrene microplastics disturb maternal-fetal immune balance and cause reproductive toxicity in pregnant mice
Polystyrene microplastics disturb maternal-fetal immune balance and cause reproductive toxicity in pregnant mice
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DOI：
10.1016/j.reprotox.2021.10.002
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发表时间：
2021-10-09
影响因子：
3.3
通讯作者：
Liu, Xiaorui
中科院分区：
文献类型：
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作者：
Hu, Jianing;Qin, Xiaoli;Liu, Xiaorui
微塑料作为一种新型的环境污染物，对人类健康的威胁已引起人们的极大关注。成功的妊娠取决于母胎免疫平衡的复杂调节，但妊娠早期暴露于聚苯乙烯MP（PS-MP）的风险仍不清楚。在这项研究中，我们将C57 BL/6交配的BALB/c小鼠暴露于PS-MP颗粒，并使用流式细胞术来探索对免疫系统的威胁。在此，同种异体交配小鼠模型显示在围着床期暴露于10 μ mPS-MP颗粒时胚胎吸收率升高。子宫微动脉数量减少，管径变细，可能导致子宫血供减少。此外，蜕膜自然杀伤细胞的百分比减少，而胎盘中的辅助性T细胞增加。此外，巨噬细胞中的M1/M2比率显著逆转为占优势的M2亚型。最后，细胞因子分泌转向免疫抑制状态。总体而言，我们的研究结果表明，PSMP有可能通过免疫紊乱对妊娠结局造成不良影响，为MP颗粒在人体内的生殖毒性研究提供了新的见解。
Microplastics (MPs), which are emerging as a new type of environmental pollutants, have raised great concerns regarding their threats to human health. A successful pregnancy depends on the sophisticated regulation of the maternal-fetal immune balance, but the risks of polystyrene MP (PS-MP) exposure in early pregnancy remain unclear. In this study, we exposed the C57BL/6-mated BALB/c mice to PS-MP particles and used the flow cytometry to explore threats towards the immune system. Herein, the allogeneic mating murine model showed an elevated embryo resorption rate with a 10 mu m PS-MP particle exposure during the peri-implantation period. Both the number and diameter of uterine arterioles decreased, which might reduce the uterine blood supply. Moreover, the percentage of decidual natural killer cells was reduced, whereas the helper T cells in the placenta increased. In addition, the M1/M2 ratio in macrophages reversed significantly to a dominant M2-subtype. Lastly, the cytokine secretion shifted towards an immunosuppressive state. Overall, our results demonstrated that PSMPs have the potential to cause adverse effects on pregnancy outcomes via immune disturbance, providing new insights into the study of reproductive toxicity of MP particles in the human body.