Development of catecholamine responsiveness in granulosa cells from preovulatory rat follicles--dependence on preovulatory luteinizing hormone surge.
Development of catecholamine responsiveness in granulosa cells from preovulatory rat follicles--dependence on preovulatory luteinizing hormone surge.
复制标题
排卵前大鼠卵泡颗粒细胞中儿茶酚胺反应性的发展——依赖于排卵前黄体生成素激增。
作者：
C. Rani;K. Nordenström;E. Norjavaara;K. Ahrén
用孕马血清促性腺激素（PMSG）注射未成熟大鼠26天，观察排卵前卵泡颗粒细胞（Gc）中儿茶酚胺（CA）反应性的形成因素。CA没有刺激整个卵泡中的环腺苷酸（cAMP）的生产之前（早晨）或之后（晚上）的排卵前促性腺激素激增的第28天，而新形成的黄体发现在第29天响应CA。从排卵前卵泡GC没有回应CA时，立即隔离后进行测试。将Gc在不含血清的Eagle's MEM中培养不同时间，随后测试CA对cAMP和类固醇生成反应的可能刺激。上午分离的Gc（AM-Gc）培养12或24 h后，对CA无反应，而晚上分离的Gc（PM-Gc）培养12 h后，对CA有明显的反应。通过全部或部分消除促性腺激素激增，使用戊巴比妥结合促黄体生成素（LH）或使用LH或促卵泡激素（FSH）的特异性抗血清，发现之前暴露于LH是PM-Gc在培养期间产生CA反应所必需的。此外，它是可能的诱导CA的AM-Gc反应，通过LH处理大鼠在体内一段时间，随后一段时间的培养。当培养过程中存在放线菌酮（5 μ g/ml）时，培养12 h的PM-Gc CA反应性的出现被取消。看来，分离的Gc必须满足以下三个条件才能产生CA反应性：1）体内暴露于LH，2）短期培养，3）活性蛋白合成。它的结论是，在生理条件下，黄体化的过程是与收购CA的反应性，这个过程取决于排卵前促性腺激素激增的LH成分。
Factors responsible for development of catecholamine (CA) responsiveness in granulosa cells (Gc) of preovulatory follicles from immature rats injected with 10 IU of pregnant mare's serum gonadotropin (PMSG) on Day 26 were studied. CA did not stimulate cyclic AMP (cAMP) production in whole follicles isolated before (morning) or after (evening) the preovulatory gonadotropin surge on Day 28, while newly formed corpora lutea found on Day 29 responded to CA. Gc from the preovulatory follicles did not respond to CA when tested immediately after isolation. Gc were cultured for various periods in Eagle's MEM without serum and subsequently tested for a possible stimulation of cAMP and steroidogenic responses by CA. In Gc from follicles isolated in the morning (AM-Gc) and cultured for 12 or 24 h, no response to CA was found, while the Gc isolated in the evening (PM-Gc) and cultured similarly for 12 h showed a marked response to CA both in stimulation of cAMP and progesterone production. By a total or partial elimination of the gonadotropin surge, using pentobarbital in combination with luteinizing hormone (LH) or using specific antisera to LH or follicle-stimulating hormone (FSH), it was found that previous exposure to LH was necessary for the PM-Gc to develop CA responsiveness during culture. Further, it was possible to induce CA responsiveness in AM-Gc by treatment of rats with LH for a short period in vivo followed by a period of culture. The appearance of CA responsiveness in PM-Gc cultured for 12 h was abolished when cycloheximide (5 micrograms/ml) was present during culture. It appears that the following three conditions have to be satisfied for the isolated Gc to develop CA responsiveness: 1) exposure to LH in vivo, 2) culture for a short period, and 3) an active protein synthesis. It is concluded that under physiological conditions the process of luteinization is associated with acquisition of responsiveness to CA and this process depends on the LH component of the preovulatory gonadotropin surge.
兔卵巢组织中肾上腺素敏感的腺苷酸环化酶活性。
影响因子：
4.8
作者：
Hunzicker-Dunn,M
通讯作者：
Hunzicker-Dunn,M