Reconnaissance of 17 beta-estradiol, 11-ketotestosterone, vitellogenin, and gonad histopathology in common carp of United States streams; potential for contaminant-induced endocrine disruption
Reconnaissance of 17 beta-estradiol, 11-ketotestosterone, vitellogenin, and gonad histopathology in common carp of United States streams; potential for contaminant-induced endocrine disruption
复制标题
美国溪流鲤鱼 17 β-雌二醇、11-酮睾酮、卵黄蛋白原和性腺组织病理学的调查；
作者：
S. Goodbred;R. Gilliom;T. Gross;Nancy Denslow;WA Bryant;T. Schoeb
侦察性类固醇激素和其他生物标志物在普通鲤鱼被用来评估是否内分泌干扰可能发生在美国河流的鱼类，以评估内分泌干扰和污染物水平之间的关系，并确定进一步研究的要求。在25个地点（647条鱼）对成年鲤鱼（通常每种性别10- 15条）的17 s-雌二醇、11-酮睾酮、卵黄蛋白原和性腺组织病理学进行了测定，这些地点代表了美国主要地区的典型环境背景。在1994年8月至12月期间收集了鱼类，这是产卵后性腺成熟的一段时间。评估的污染物是有机氯农药和多氯联苯在组织中;邻苯二甲酸酯，苯酚，多环芳烃在床沉积物;和水中溶解的农药。两种性别的类固醇激素平均部位浓度跨越两个数量级。没有显着的区域差异，类固醇激素检测男性，但女性从北方和南部的中大陆显着不同，从其他地区的国家在一个或两个激素。在所有区域内，两性的一种或两种激素在网站之间存在显着差异。生物标志物与污染物之间的相关系数大多为负。与生物标志物具有显著相关性（a=0.05）的污染物是有机氯农药、酚类和溶解性农药。最强的模式共同的男性和女性之间的激素比例（E2/11-KT）和溶解的农药呈负相关。生物标志物的显着站点间差异，以及生物标志物和污染物之间存在显着相关性，证明某些溪流中的鱼类可能正在经历内分泌干扰。需要改进信息，以评估内分泌干扰是否确实发生，以及是否对鲤鱼或其他物种的个体或种群产生生殖影响。今后的研究应转向对较少的场地进行更深入的研究，包括参考场地和受污染场地，以解决这些额外的问题。
A reconnaissance of sex steroid hormones and other biomarkers in common carp was used to assess whether endocrine disruption may be occurring in fish in United States streams, to evaluate relations between endocrine disruption and contaminant levels, and to determine requirements for further studies. 17s-estradiol, 11-ketotestosterone, vitellogenin, and gonadal histopathology were measured in adult carp (usually 10--15 for each sex) at 25 sites (647 fish), representing a wide range of environmental settings typical of major regions of the nation. Fish were collected during August--December 1994, a period of gonadal maturation after spawning. Contaminants evaluated were organochlorine pesticides and polychlorinated biphenyls in tissue; phthalates, phenols, and polycyclic aromatic hydrocarbons in bed sediment; and dissolved pesticides in water. Mean site concentrations of steroid hormones spanned two orders of magnitude for both sexes. No significant regional differences in steroid hormones were detected for males, but females from the Northern and Southern Midcontinent were significantly different from other regions of the country in one or both hormones. Within all regions there were significant differences between sites in one or both hormones for both sexes. Most correlation coefficients between biomarkers and contaminants were negative. Contaminants that had significant (a=0.05) correlations with biomarkers were organochlorine pesticides, phenols, and dissolved pesticides. The strongest pattern common to both males and females was a negative correlation between the hormone ratio (E2/11-KT) and dissolved pesticides. The significant site-to-site differences in biomarkers, and the presence of significant correlations between biomarkers and contaminants, are evidence that fish in some streams may be experiencing endocrine disruption. Improved information is needed to evaluate whether endocrine disruption is actually occurring and if there are reproductive effects on individual or populations of carp or other species. Future studies should shift to more intensive study of fewer sites, including reference and contaminated sites, in order to address these additional questions.
影响因子：
--
作者：
G. L. Peterson
通讯作者：
G. L. Peterson