Overexpression of CYP2E1 enhances sensitivity of HepG2 cells to Fas-mediated cytotoxicity
Overexpression of CYP2E1 enhances sensitivity of HepG2 cells to Fas-mediated cytotoxicity
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CYP2E1 过表达增强 HepG2 细胞对 Fas 介导的细胞毒性的敏感性
DOI：
10.4161/cbt.7.8.6283
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发表时间：
2008
影响因子：
3.6
通讯作者：
Gao
中科院分区：
文献类型：
--
作者：
Q. Yan;Jianguo Shi;Feng Zhang;Qing Zhao;X. Pang;R. Chen;Pei;Qin;Zhe Wang;Gao
肝细胞癌（HCC）对Fas介导的细胞凋亡具有抵抗性。本研究旨在探讨CYP 2 E1过表达对肝癌细胞对Fas介导的细胞毒作用敏感性的影响。用腺病毒介导的CYP 2 E1感染肝癌细胞株HepG 2，以增强细胞色素P450 2 E1的表达，然后用低毒性剂量的重组人Fas配体（FasL，0.5ng/ml）和放线菌素D（ActD，125 ng/ml）处理肝癌细胞。Fas在HepG 2细胞中有高水平表达。不同处理后其蛋白质含量和分布基本不变。与对照组相比，表达CYP 2 E1的HepG 2细胞对FasL + Act D更敏感。CYP 2 E1抑制剂二烯丙基二硫（DAS）可显著抑制该敏感性（P
Hepatocellular carcinoma (HCC) has been reported to be resistant to Fas-mediated apoptosis. In present study, experiments were conducted to investigate the potential effects of CYP2E1 overexpression on susceptibility of HCC to Fas-mediated cytotoxicity. HCC cell line HepG2 was infected with Ad-CYP2E1 to enhance the expression of CYP2E1, followed by treatment with low toxic dose of recombinant human Fas ligand (FasL, 0.5ng/ml) in the presence of Actinomycin D (Act D, 125ng/ml). High level of Fas expression was found in HepG2 cells. Its protein level and distribution kept unchanged after different treatments. Compared with control, CYP2E1 expressed HepG2 cells were more sensitive to FasL plus Act D. The sensitivity was elevated in a multiplicity of infection (m.o.i)-dependent manner, which was dramatically suppressed by CYP2E1 inhibitor diallyl disulfide (DAS) (P
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影响因子：
3.6
作者：
Chen, Q;Cederbaum, AI
通讯作者：
Cederbaum, AI
水杨酸钠可增加 HepG2 细胞和培养的大鼠肝细胞中 CYP2E1 的水平并增强花生四烯酸的毒性。
影响因子：
3.6
作者：
Wu,D;Cederbaum,AI
通讯作者：
Cederbaum,AI
影响因子：
7.4
作者：
Wang, Xiaodong;Cederbaum, Arthur I.
通讯作者：
Cederbaum, Arthur I.
DOI：
--
发表时间：
1985-09
期刊：
Drug metabolism and disposition: the biological fate of chemicals
影响因子：
--
作者：
L. A. Reinke;M. J. Moyer
通讯作者：
L. A. Reinke;M. J. Moyer