Alterations in Peripheral B Cell Subsets Correlate with the Disease Severity of Human Glaucoma.
Alterations in Peripheral B Cell Subsets Correlate with the Disease Severity of Human Glaucoma.
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DOI：
10.2147/jir.s329084
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发表时间：
2021
影响因子：
4.5
通讯作者：
Lu F
中科院分区：
文献类型：
--
作者：
Yu L;Chen Y;Xu X;Dong Q;Xiu W;Chen Q;Wang J;He C;Ye J;Lu F
青光眼是一组视网膜神经退行性疾病，可导致不可逆的视力损害。本病发病机制复杂。研究表明，免疫系统参与青光眼的神经退行性过程。越来越多的证据表明自身抗体在青光眼的发病机制中起着至关重要的作用。然而，关注B细胞，青光眼中的抗体产生细胞令人惊讶地有限。从44名青光眼患者（38名原发性闭角型青光眼（PACG）和6名原发性开角型青光眼（POAG））和36名年龄匹配的健康供体（HD）收集新鲜外周血样品。进行密度梯度离心以获得外周血单个核细胞（PBMC）。流式细胞术测定B细胞表型。根据视野平均偏差（MD）确定青光眼的严重程度。在这项研究中，我们证明，总B细胞显着增加青光眼患者相比，HD。接下来，我们检查了青光眼中不同B细胞亚群的变化。研究发现，青光眼患者抗体分泌细胞（ASC）/浆母细胞（幼稚）和CD 19 + CD 27 − IgD−双阴性（DN）亚群的频率显著增加，但CD 27 + IgD+未转换记忆区室的频率降低。值得注意的是，我们发现根据临床严重程度，CD 27 − IgD− DN B细胞的增量显著放大。我们首次证明了外周B细胞亚群的改变，并揭示了一种新发现的促炎性CD 27 − IgD− DN亚群与青光眼临床特征的相关性，这表明这些B细胞亚群可以作为监测青光眼患者疾病进展的潜在生物标志物。
Glaucoma is a group of retinal neurodegenerative diseases causing irreversible visual impairment. The pathogenesis of this disease is complicated. Studies have shown that the immune system is involved in the neurodegenerative process of glaucoma. There are continuous evidences that autoantibodies play a crucial role in the pathogenesis of glaucoma. However, focuses on B cells, the antibody-producing cells in glaucoma are surprisingly limited. Fresh peripheral blood samples were collected from 44 glaucoma patients (38 with primary angle-closure glaucoma (PACG) and 6 with (primary open-angle glaucoma POAG)) and 36 age-matched healthy donors (HD). Density gradient centrifugation was performed to obtain peripheral blood mononuclear cells (PBMC). Flow cytometry was performed to determine B cell phenotypes. The severity of glaucoma was determined based on the mean deviation (MD) of visual field. In this study, we demonstrated that total B cells was significantly increased in glaucoma patients compared to HD. Next, we checked changes of different B cell subsets in glaucoma. Glaucoma patients were found to have a significant increase in the frequencies of antibody-secreting cells (ASC)/plasmablasts, naïve, and CD19+ CD27− IgD− double negative (DN) subpopulations, but a decrease in the CD27+ IgD+ unswitched memory compartment. Notably, we found that the increment of CD27− IgD− DN B cells was significantly magnified according to the clinical severity. We demonstrate, for the first time, that peripheral B cell subsets are altered and unveil the correlation of a newly identified pro-inflammatory CD27− IgD− DN subset with clinical features of glaucoma, suggesting that these B cell subsets could serve as potential biomarkers to monitor the disease progression of glaucoma patients.