The Human RC3 Gene Homolog, NRGN Contains a Thyroid Hormone-Responsive Element Located in the First Intron* * This work was supported by grants from Fundación Ramon Areces and DGICYT (PM95-0019).
The Human RC3 Gene Homolog, NRGN Contains a Thyroid Hormone-Responsive Element Located in the First Intron* * This work was supported by grants from Fundación Ramon Areces and DGICYT (PM95-0019).
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人类 RC3 基因同源物 NRGN 包含位于第一个内含子的甲状腺激素反应元件* * 这项工作得到了 Ramon Areces 基金会和 DGICYT (PM95-0019) 的资助。
作者：
Cruz Martinez de Arrieta;B. Morte;A. Coloma;J. Bernal
NRGN是神经元特异性大鼠RC3/神经粒蛋白基因的人类同源物。该基因编码突触后78个氨基酸的蛋白激酶底物，在缺乏钙的情况下结合钙调蛋白，这与树突棘的形成和突触的可塑性有关。在大鼠脑中，在发育和成年动物的特定神经元亚群中，RC3受甲状腺激素控制。为了评估人类基因是否也是甲状腺激素的靶标，我们在NRGN克隆的横跨整个基因的基因组片段中寻找t3应答元件。标记的DNA片段与T3受体（T3R）和9-顺式维甲酸受体孵育，并用抗T3R抗体进行免疫沉淀。受体结合位点定位于第一个内含子，位于转录起始点下游3000 bp处。足迹分析显示，序列GGATTAAATGAGGTAA与共识的直接重复（DR4）型t3响应元件密切相关。该序列结合t3r -9-顺式视网膜蛋白…
NRGN is the human homolog of the neuron-specific rat RC3/neurogranin gene. This gene encodes a postsynaptic 78-amino acid protein kinase substrate that binds calmodulin in the absence of calcium, and that has been implicated in dendritic spine formation and synaptic plasticity. In the rat brain RC3 is under thyroid hormone control in specific neuronal subsets in both developing and adult animals. To evaluate whether the human gene is also a target of thyroid hormone we have searched for T3-responsive elements in NRGN cloned genomic fragments spanning the whole gene. Labeled DNA fragments were incubated with T3 receptors (T3R) and 9-cis-retinoic acid receptors and immunoprecipitated using an anti T3R antibody. A receptor-binding site was localized in the first intron, 3000 bp downstream from the origin of transcription. Footprinting analysis revealed the sequence GGATTAAATGAGGTAA, closely related to the consensus T3-responsive element of the direct repeat (DR4) type. This sequence binds the T3R-9-cis-retin...
神经元特异性基因 RC3（神经粒蛋白）的启动子区域和侧翼序列的表征。
DOI：
10.1016/0169-328x(94)90002-7
发表时间：
1994
期刊：
Brain research. Molecular brain research
影响因子：
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作者：
Iñiguez,MA;Morte,B;Rodriguez-Peña,A;Muñoz,A;Gerendasy,D;Sutcliffe,JG;Bernal,J
通讯作者：
Bernal,J