Frequency of alloreactive cytotoxic T cell precursors in the mouse thymus and spleen during ontogeny.
Frequency of alloreactive cytotoxic T cell precursors in the mouse thymus and spleen during ontogeny.
复制标题
个体发育期间小鼠胸腺和脾脏中同种反应性细胞毒性 T 细胞前体的频率。
DOI：
10.1097/00007890-197911000-00006
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发表时间：
1979
期刊：
影响因子：
6.2
通讯作者：
R. Ceredig
中科院分区：
文献类型：
--
作者：
R. Ceredig
描述了一种混合淋巴细胞培养系统，其中来自CBA（H-2k）小鼠的非常少量的胸腺或脾细胞与同种异体（H-2d）细胞一起培养，并且通过从标记的靶细胞释放51 Cr来确定单个培养物的所得细胞介导的淋巴溶解（CML）反应。使用这种培养系统和胸腺和脾细胞从CBA小鼠的不同年龄，有限稀释分析CML的反应性进行了估计，从而作出了同种异体反应（抗H-2d）细胞毒性T淋巴细胞前体细胞（CTL-P）在这两个器官在个体发育的频率。胎儿胸腺细胞不含可检测到的CTL-P或能够抑制体外成人脾细胞CTL-P反应的细胞。在1日龄小鼠胸腺中首次检测到CTL-P。到4周龄时，它们的频率从最初的1/5 × 105个细胞的低值增加到1/6.8 × 104个细胞，每个胸腺有2，700个抗H-2d CTL-P。9月龄小鼠的胸腺仅含有280个CTL-P，频率为1/2 × 105个细胞。脾脏的CML反应性在3日龄时首次检测到。脾脏中CTL-P的频率最初较低，但随后CTL-P逐渐蓄积，直至至少9月龄时频率为1/3.5 × 103个细胞。这些结果表明胸腺和脾脏CML反应性之间存在直接关系，并且最初在胸腺中产生的CTL-P迁移并在脾脏中变成CTL-P。
A mixed lymphocyte culture system is described in which very small numbers of thymus or spleen cells from CBA (H-2k) mice are cultured with allogeneic (H-2d) cells, and the resultant cell-mediated lympholysis (CML) response of individual cultures is determined by 51Cr release from labeled target cells. Using this culture system and thymus and spleen cells from CBA mice of various ages, limit dilution analysis of CML responsiveness was carried out and estimates were thereby made of the frequency of alloreactive (anti-H-2d) cytotoxic T lymphocyte precursors (CTL-P) in both organs during ontogeny. Fetal thymus cells contained no detectable CTL-P or cells capable of suppressing CTL-P responses from adult spleen cells in vitro. CTL-P were first detected in the thymus of 1-day-old mice. By 4 weeks of age their frequency had increased from an initial low value of 1/5 × 105 cells to 1/6.8 × 104 cells, and there were 2,700 anti-H-2d CTL-P/thymus. The thymus of the 9-month-old mouse contained only 280 CTL-P at a frequency of 1/2 × 105 cells. CML responsiveness of the spleen was first detected at 3 days of age. The frequency of CTL-P in the spleen was initially low, but there followed a progressive accumulation of CTL-P until at least 9 months of age when the frequency was 1/3.5 × 103 cells. These results suggest a direct relationship between thymus and spleen CML responsiveness and that CTL-P initially generated in the thymus migrate and become CTL-P in the spleen.