Modulation of radiation-induced strand break repair by cisplatin in mammalian cells
Modulation of radiation-induced strand break repair by cisplatin in mammalian cells
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DOI：
10.1080/095530098141735
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发表时间：
1998-07-01
影响因子：
2.6
通讯作者：
Raaphorst, GP
中科院分区：
文献类型：
--
作者：
Dolling, JA;Boreham, DR;Raaphorst, GP
目的：探讨顺铂-DNA加合物对电离辐射诱导的人皮肤成纤维细胞DNA损伤的修复作用，并确定顺铂对DNA修复抑制的持久性。材料和方法：顺铂处理的正常人成纤维细胞（AG 1522）暴露在4 Gy Co-60 γ射线下，在生长许可条件下检测辐射诱导损伤的修复。DNA损伤通过DNA解绕荧光分析（FADU）和细胞分裂阻断微核测定测定。结果：在未经顺铂预处理的细胞中，dr Gy辐射引起的链断裂的重新连接似乎是双相的，最初的快速组分（长达15分钟的修复时间）随后是较慢的组分，并在90分钟内完成。照射前立即使用顺铂治疗（10 μ g/ml, 30 min）对快速再连接成分没有影响，但抑制慢速成分(p
Purpose: To investigate the repair of ionizing radiation-induced DNA lesions in human skin fibroblasts in the presence of cisplatin-DNA adducts and to determine the persistence of DNA repair inhibition by cisplatin.Materials and methods: Normal human fibroblasts (AG 1522) treated with cisplatin were exposed to 4 Gy Co-60 gamma-radiation and assayed for repair of radiation-induced damage under growth-permissive conditions. DNA damage was measured by the fluorescence analysis of DNA unwinding (FADU) and cytokinesis-blocked micronucleus assays.Results: Rejoining of strand breaks caused by dr Gy radiation in cells without cisplatin pre-treatment appeared to be biphasic with an initial fast component (up to 15 min of repair time) followed by a slower component, and was completed by 90 min. Cisplatin treatment (10 mu g/ml, 30 min) immediately before irradiation had no effect on the fast rejoining component, but inhibited the slow component (p