DEVELOPMENTAL STAGE SPECIFICITY OF THE LYMPHOID V(D)J RECOMBINATION ACTIVITY
DEVELOPMENTAL STAGE SPECIFICITY OF THE LYMPHOID V(D)J RECOMBINATION ACTIVITY
复制标题
DOI：
10.1101/gad.1.8.751
复制
发表时间：
1987-10-01
影响因子：
10.5
通讯作者：
GELLERT, M
中科院分区：
文献类型：
--
作者：
LIEBER, MR;HESSE, JE;GELLERT, M
我们检查了许多源自淋巴或非淋巴谱系的细胞系中免疫球蛋白基因 V(D)J 重组活性的水平。我们采用的测定方法使用染色体外 DNA 作为底物，从而避免了与使用染色体整合底物相关的困难。 B 淋巴细胞发育过程中重组活性降低。它在 B 细胞定向分化的最早阶段最高，然后逐渐下降，在成熟 B 细胞阶段达到不可检测的水平。该活性也存在于骨髓细胞的多能祖细胞和前 T 细胞中，但不存在于成熟 T 细胞中。在几种非造血细胞系中没有发现活性。仅在真核细胞中复制的底物分子之间观察到重组。讨论了该结果的几种可能的解释。
We have examined the level of immunoglobulin gene V(D)J recombination activity in a number of cell lines derived from lymphoid or nonlymphoid lineages. The assay we employed uses extrachromosomal DNA as substrate and thereby avoids difficulties associated with the use of chromosomally integrated substrates. The recombination activity decreases during B-lymphoid development. It is highest at the earliest stages of committed B-cell differentiation and then falls progressively, reaching undetectable levels at the mature B-cell stage. The activity is also present in multipotential progenitors of myeloid cells and in pre-T cells but not mature T cells. No activity was found in several nonhematopoietic cell lines. Recombination was seen only among substrate molecules which had replicated in the eukaryotic cells. Several possible interpretations of this result are discussed.