Epitope characterization of MUC1 antibodies
Epitope characterization of MUC1 antibodies
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DOI：
10.1159/000056504
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发表时间：
1998-01-01
期刊：
影响因子：
--
通讯作者：
Nilsson, O
中科院分区：
文献类型：
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作者：
Blockzjil, A;Nilsson, K;Nilsson, O
提交给ISOBM TD-4研讨会的56株单抗通过与MUC1阳性细胞株(ZR75-1)和MUC1阴性细胞株(LS174T)的酶联免疫吸附试验(ELISA)鉴定其特异性，结合与MUC1！串联重复区域相对应的20肽。用IsoBM TD-4单抗作为捕捉单抗，对ZR75-1细胞中可溶性MUC1抗原的识别进行了双位点EIA检测。研究表明，35个提交的抗体识别了MUC1串联重复区域中表达的表位，其中30个识别表位位于串联重复序列的免疫优势区(PDTRPAP)内，对ZR75-1细胞中可溶性MUC1的识别表明，另外10个MAb识别了来自ZR75-1细胞的MUC1串联重复区域之外的表位，或者，抗体识别表位取决于MUC1串联重复序列的糖基化。6株单抗仅与MUC1阴性细胞系LS 174T反应，因此很可能不识别MUC1的特异性表位。
The specificities of the 56 MAbs submitted to the ISOBM TD-4 Workshop were characterized by ELISA assays against MUC1-positive (ZR75-1) and MUC1-negative cell lines (LS174T), binding to a 20-mer peptide corresponding to the tandem repeat region of MUC1! apoprotein, and Pepscan analysis of overlapping 9-mer peptides corresponding to the tandem repeat region of MUC1., The recognition of soluble MUC1 antigen from ZR75-1 cell line was also tested in two-site EIA using the ISOBM TD-4 MAb as catching MAb, The studies indicated that 35 of the submitted antibodies recognized epitopes expressed in the MUC1 tandem repeat region, and that 30 of these recognized epitopes within the immunodominant region (PDTRPAP) of the tandem repeat, Recognition of soluble MUC1 from ZR75-1 cell line indicated that additional 10 MAb recognized epitopes 'outside' the tandem repeat region of MUC1 from ZR75-1 cell line, or that the antibodies recognized epitopes depending on the glycosylation of the MUC1 tandem repeat. Six MAbs reacted only with the MUC1-negative cell line LS 174T, and thus most likely did not recognize epitopes specific for MUC1.