Molecular cloning and gene expression of Foxl2 in the frog Rana rugosa
Molecular cloning and gene expression of Foxl2 in the frog Rana rugosa
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DOI：
10.1016/j.ygcen.2008.08.013
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发表时间：
2008-11-01
影响因子：
2.7
通讯作者：
Nakamura, Masahisa
中科院分区：
文献类型：
--
作者：
Oshima, Yuki;Uno, Yoshinobu;Nakamura, Masahisa
Foxl 2是一种转录因子，在哺乳动物和鱼类雌性性腺的早期发育调控中起着至关重要的作用。然而，关于它在两栖动物卵巢分化中的作用知之甚少。在这项研究中，我们从青蛙Rana rugosa和非洲爪蟾Xenopus laevis的卵巢中分离了Foxl 2 cDNA，并检测了其在Rana rugosa性腺性别分化过程中的表达。皱纹。来自脊椎动物的已知Foxl 2序列的比对显示Foxl 2开放阅读框和蛋白质序列，特别是叉头结构域和C-末端区域与其他脊椎动物序列的高度同一性。在不同的成体组织中，Foxl 2在卵巢中的表达水平最高。实时荧光定量RT-PCR分析表明，Foxl 2在红腹锦鸡性腺分化过程中的表达呈性二态性。皱纹。此外，Foxl 2在卵巢卵母细胞周围的体细胞中被免疫化学检测到，它促进了R。在A6细胞中进行的荧光素酶启动子测定中，rugosa CYP 19转录。FISH分析发现Fox 12为常染色体基因。综上所述，Foxl 2可能在卵巢分化中起重要作用。可能通过调控CYP 19的表达。在雌性性腺中上调Foxl 2表达的因子仍有待鉴定。(c)2008年爱思唯尔公司All rights reserved.
Foxl2 is a transcription factor that plays a crucial role in the regulation of the early development of the female gonad in mammals and fish. However, little is known regarding its role in ovarian differentiation in amphibians. In this study, we isolated a Foxl2 cDNA from the ovary of the frog Rana rugosa and Xenopus laevis and examined its expression during gonadal sex differentiation in R. rugosa. Alignment of known Foxl2 sequences from vertebrates showed high identity of the Foxl2 open reading frame and protein sequences, in particular the forkhead domain and C-terminal region, with other vertebrate sequences. Among different adult tissues, Foxl2 was expressed at its highest level in the ovary. Real-time RT-PCR analysis showed that Foxl2 expression was sexually dimorphic during gonadal sex differentiation in R. rugosa. In addition, Foxl2, which was detected immunochemically in somatic cells surrounding oocytes in the ovary, promoted R. rugosa CYP19 transcription in luciferase promoter assays conducted in A6 cells. We also found by FISH analysis that Foxl2 was an autosomal gene. Altogether, these results suggest that Foxl2 probably plays a very important role in ovarian differentiation of R. rugosa by possibly regulating CYP19 expression. The factor that upregulates Foxl2 expression in female gonads Still remains to be identified. (c) 2008 Elsevier Inc. All rights reserved.