Activity of horseradish peroxide adsorbed on radio frequency glow discharge-treated polymers.
Activity of horseradish peroxide adsorbed on radio frequency glow discharge-treated polymers.
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射频辉光放电处理的聚合物上吸附的辣根过氧化物的活性。
DOI：
10.1163/156856294x00734
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Hoffman,AS
中科院分区：
文献类型：
--
作者：
Chen,JP;Kiaei,D;Hoffman,AS
Horseradish peroxidase (HRP) has been used as a model enzyme in this study of its physical adsorption and residual enzyme activity on radio frequency glow discharge (RFGD)-treated polymers. The specific enzymatic activity of HRP adsorbed on different surfaces was assumed to be an indication of the extent of its conformational alterations on the surfaces. The surfaces studied were poly (ethylene terephthalate) (PET), polytetrafluoroethylene (PTFE), and tetrachloroethylene and tetrafluoroethylene glow discharge-treated PET, abbreviated as TCE/PET and TFE/PET. All surfaces were characterized by electron spectroscopy for chemical analysis (ESCA) and liquid contact angles in air. HRP adsorbs more strongly onto the two discharge-treated surfaces than onto the untreated polymers, as evidenced by the lower amount of HRP eluted by sodium dodecyl sulfate (SDS) from the treated polymers. For example, seventy percent of the HRP adsorbed on TCE/PET or TFE/PET remains on the surface after overnight elution with a 1% solution of SDS. In contrast, untreated PET and PTFE each retains only c. 20% of the absorbed enzyme. The enzymatic activity of HRP adsorbed on the different surfaces was studied using hydrogen peroxide (H2O2) as the substrate. HRP adsorbed on the higher energy surfaces, PET and TCE/PET, retains significantly more activity than the HRP adsorbed on the lower energy surfaces, PTFE and TFE/PET which appear to destroy rapidly almost all of the activity of HRP after it adsorbs. HRP adsorbed on TCE/PET is relatively more stable over time than HRP adsorbed on PET or free HRP in solution. (For example, only 45% of the specific enzymatic activity of HRP adsorbed on TCE/PET was lost after 3 h of storage in phosphate buffer at 37°C, while 70% of that adsorbed on PET was lost.) In summary, when HRP is adsorbed on TCE/PET, it is very tightly bound, and yet it maintains a significant fraction of its initial specific activity and also retains this activity for 3 h in phosphate buffer at 37°C. Thus, tenacious physical adsorption of proteins such as enzymes on TCE glow discharge-treated surfaces may have potential as a new method of immobilization of such molecules, for uses in biosensors, diagnostics, bioseparations, cell culture and bioreactors.
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基态和激发态大分子构象分析的量子力学方法
DOI：
--
发表时间：
1969
期刊：
影响因子：
--
作者：
R. Hoffmann;A. Imamura
通讯作者：
A. Imamura
DOI：
10.2210/pdb3dfr/pdb
发表时间：
1982-11
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J. Bolin;D. Filman;D. Matthews;R. Hamlin;J. Kraut
通讯作者：
J. Bolin;D. Filman;D. Matthews;R. Hamlin;J. Kraut
工程化的亚基间二硫键增强了 lambda 阻遏物 N 末端结构域的稳定性和 DNA 结合。
影响因子：
2.9
作者：
Sauer,RT;Hehir,K;Stearman,RS;Weiss,MA;Jeitler-Nilsson,A;Suchanek,EG;Pabo,CO
通讯作者：
Pabo,CO
来自 Centruroides sculpturatus Ewing 的变体 3 蝎子神经毒素的结构，以 1.8 A 分辨率精制。
影响因子：
5.6
作者：
Almassy,RJ;Fontecilla-Camps,JC;Suddath,FL;Bugg,CE
通讯作者：
Bugg,CE
以 1.4 A 分辨率精修的不同配体状态下的赤十字蛋白的结构。
影响因子：
5.6
作者：
W. Steigemann;E. Weber
通讯作者：
E. Weber