IMMUNOHISTOCHEMICAL ANALYSIS OF HUMAN UTERINE ESTROGEN AND PROGESTERONE RECEPTORS THROUGHOUT THE MENSTRUAL-CYCLE
IMMUNOHISTOCHEMICAL ANALYSIS OF HUMAN UTERINE ESTROGEN AND PROGESTERONE RECEPTORS THROUGHOUT THE MENSTRUAL-CYCLE
复制标题
DOI：
10.1210/jcem-67-2-334
复制
发表时间：
1988-08-01
影响因子：
5.8
通讯作者：
MCCARTY, KS
中科院分区：
文献类型：
--
作者：
LESSEY, BA;KILLAM, AP;MCCARTY, KS
应用特异性抗雌激素受体单克隆抗体，对33例不同月经周期妇女子宫组织中雌激素受体（ER）和孕激素受体（PgR）进行了免疫组化研究。免疫组织化学定位定量染色强度和组织分布在腺上皮，基质，和子宫肌层，并与标准的配体结合试验的结果进行了比较。在所有样品中，ER和PgR定位于靶细胞的细胞核内。ER和PgR的最高浓度发生在月经周期的中期至晚期增殖期。ER含量在整个分泌期呈下降趋势。与此相反，PgR的内容经历了出乎意料的复杂和不同步的波动在月经周期的分泌期。具体地说，腺上皮PgR含量减少，而基质和子宫肌层保持了显着的PgR含量。PgR和可能的ER在子宫内的不同细胞类型中是不一致的。通过受体含量的改变进行功能分离可能是靶组织激素依赖性生长和分化的重要机制。
Estrogen receptors (ER) and progesterone receptors (PgR) were studied immunohistochemically using specific antireceptor monoclonal antibodies in uterine tissue samples from 33 women in various stages of the menstrual cycle. Immunohistochemical localization was quantified as to intensity of staining and tissue distribution in glandular epithelium, stroma, and myometrium, and the results were compared with those of standard ligand binding assays. In all samples ER and PgR localized within the nuclei of target cells. The maximal concentrations of ER and PgR occurred in the mid- to late proliferative phase of the menstrual cycle. ER content declined throughout the secretory phase. In contrast, PgR content underwent unexpectedly complex and dyssynchronous fluctuations during the secretory phase of the menstrual cycle. Specifically, the glandular epithelium had diminished PgR content, while the stroma and myometrium maintained a significant PgR content. PgR and perhaps ER are not concordant in different cell types within the uterus. Segregation of function through alteration of receptor content may be an important mechanism in steroid-dependent growth and differentiation of target tissues.