Femtosecond laser photodisruption of human trabecular meshwork: an in vitro study
Femtosecond laser photodisruption of human trabecular meshwork: an in vitro study
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DOI：
10.1016/j.exer.2005.02.001
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发表时间：
2005-09-01
影响因子：
3.4
通讯作者：
Edward, DP
中科院分区：
文献类型：
--
作者：
Toyran, S;Liu, YM;Edward, DP
这项体外研究的目的是测试使用飞秒 (fsec) 激光脉冲造瘘人类小梁网 (TM) 的可行性，并确定创建消融通道所需的最小曝光时间和能量剂量。角巩膜边缘取自用于穿透性角膜移植术的组织。在 Optisol-GS 中水合的四毫米组织条用于通过将具有不同脉冲能量（7.2 和 14.4 μJ）和曝光时间（0.25、0.5、1 和 2 秒）的钛宝石激光束（45 fsec、1 kHz、800 nm）聚焦在 TM 的内表面上，在人体 TM 中形成部分厚度的瘘管。使用超快钛蓝宝石光源，通过多光子显微镜获得病变的双光子图像。此外，通过光学显微镜检查固定组织的切片。根据苏木精和伊红 (H&E) 染色切片确定病变的直径和长度，并根据双光子图像评估病变周围的胶原结构。所有选定的时间点（0.25 秒除外）和能量均实现了 TM 所需的光破坏。用 0.5 秒/14.4 μJ 照射创建的切口似乎是最合适的，因为它们能够实现一致的全层小梁消融。在相同脉冲能量下，1秒/14.4μJ/脉冲和2秒/14.4μJ/脉冲产生的切口比在更短时间点产生的切口更深。较长的曝光时间和较高的脉冲能量通常变化更大，并且与更深、更大的切口和轻微的附带损伤相关。我们的结果表明，通过适当的曝光时间和脉冲能量，fsec 光致破裂可用于在人类 TM 中产生损伤，而不损伤周围组织。这项研究表明 fsec 激光治疗未来可能具有青光眼手术治疗的潜力。 (c) 2005 Elsevier Ltd. 保留所有权利。
The goal of this in vitro study was to test the feasibility of using femtosecond (fsec) laser pulses to fistulize the human trabecular meshwork (TM), and to determine the minimum exposure time and energy dosage needed to create an ablation channel. Corneo-scleral rims were obtained from tissue used for penetrating keratoplasty. Four millimeter tissue strips hydrated in Optisol-GS were used to create partial thickness fistulas in the human TM by focusing a Ti:Sapphire laser beam (45 fsec, 1 kHz, 800 nm) with various pulse energies (7.2 and 14.4 mu J) and exposure times (0.25, 0.5, 1, and 2 sec) on the inner surface of the TM. Two-photon images of the lesions were obtained with a multiphoton microscope, using an ultrafast Ti:Sapphire light source. In addition, sections of fixed tissue were examined by light microscopy. Diameters and lengths of the lesions were determined from the hematoxylin and eosin (H&E) stained sections, and the collagen structure surrounding the lesion was evaluated from the two-photon images.All selected time points (except for 0.25 sec) and energies achieved the desired photodisruption of the TM. Incisions created with 0.5 sec/14.4 mu J irradiation appeared to be the most suitable because they were able to achieve consistent full thickness trabecular ablation. Incisions created at 1 sec /14.4 mu J/pulse and 2 sec/14.4 mu J/pulse were deeper than those at shorter time points with the same pulse energy. Longer exposure times and higher pulse energies were usually more variable and associated with deeper and larger incisions and slight collateral damage.Our results indicate that, with appropriate exposure time and pulse energy, fsec photodisruption can be employed to create lesions in the human TM without damaging the surrounding tissues. This study demonstrates that fsec laser treatments may have future potential for the surgical treatment of glaucoma. (c) 2005 Elsevier Ltd. All rights reserved.