Long helical filaments are not seen encircling cells in electron cryotomograms of rod-shaped bacteria.
Long helical filaments are not seen encircling cells in electron cryotomograms of rod-shaped bacteria.
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DOI：
10.1016/j.bbrc.2011.03.062
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发表时间：
2011-04-22
影响因子：
3.1
通讯作者：
Jensen GJ
中科院分区：
文献类型：
--
作者：
Swulius MT;Chen S;Jane Ding H;Li Z;Briegel A;Pilhofer M;Tocheva EI;Lybarger SR;Johnson TL;Sandkvist M;Jensen GJ
How rod-shaped bacteria form and maintain their shape is an important question in bacterial cell biology. Results from fluorescent light microscopy have led many to believe that the actin homolog MreB and a number of other proteins form long helical filaments along the inner membrane of the cell. Here we show using electron cryotomography of six different rod-shaped bacterial species, at macromolecular resolution, that no long (>80 nm) helical filaments exist near or along either surface of the inner membrane. We also use correlated cryo-fluorescent light microscopy (cryo-fLM) and electron cryo-tomography (ECT) to identify cytoplasmic bundles of MreB, showing that MreB filaments are detectable by ECT. In light of these results, the structure and function of MreB must be reconsidered: instead of acting as a large, rigid scaffold that localizes cell-wall synthetic machinery, moving MreB complexes may apply tension to growing peptidoglycan strands to ensure their orderly, linear insertion.
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