CROSS-REACTIVE RECOGNITION BY ANTIGEN-SPECIFIC, MAJOR HISTOCOMPATIBILITY COMPLEX-RESTRICTED T-CELLS OF A MITOGEN DERIVED FROM MYCOPLASMA-ARTHRITIDIS IS CLONALLY EXPRESSED AND I-E RESTRICTED
CROSS-REACTIVE RECOGNITION BY ANTIGEN-SPECIFIC, MAJOR HISTOCOMPATIBILITY COMPLEX-RESTRICTED T-CELLS OF A MITOGEN DERIVED FROM MYCOPLASMA-ARTHRITIDIS IS CLONALLY EXPRESSED AND I-E RESTRICTED
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DOI：
10.1002/eji.1830160706
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发表时间：
1986-07-01
影响因子：
5.4
通讯作者：
HODES, RJ
中科院分区：
文献类型：
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作者：
LYNCH, DH;COLE, BC;HODES, RJ
为了确定主要组织相容性复合体（MHC）编码的限制性元件在T细胞识别其主要抗原时是否会影响其被MAS（关节炎支原体的可溶性产物）交叉反应刺激的能力，我们测试了一组克隆的、可溶性抗原特异性的I-A和i -e限制性T细胞对MAS的交叉反应识别和反应能力。最初的研究表明，所有被测试的克隆T细胞都能够在基因e - α、e - β存在的情况下对MAS产生反应。-表达（I-E+），但不表达e - α和e - β。-非表达（I-E-）副细胞（AC）。然而，随后的研究表明，大多数这些T细胞克隆在存在I-E+ AC的情况下对MAS产生增殖反应的能力依赖于作为AC源的辐照脾细胞中Lyt-1+2- T细胞的存在。当使用T细胞耗尽、I-E+脾脏细胞群或I-E+抗原呈递系（WEHI-5）作为AC来源时，34个被测试的克隆中只有6个被发现对MAS有直接反应。在MAS I-E产品刺激后，这些MAS反应性T细胞克隆能够“招募”旁观者T细胞增殖。最后，给定T细胞克隆对MAS加I-E产物的反应能力似乎不受该克隆在对其他抗原的反应中使用的限制性元件的影响，因为两种限制i - a的T细胞克隆对MAS加I-E的反应比例相同。因此，所提供的数据表明，i - e限制性T细胞对MAS的反应性是T细胞的一种克隆表达特性，它独立于其传统的抗原特异性和MHC限制性模式。
In order to determine whether or not the major histocompatibility complex (MHC)-encoded restriction element used by a T cell in the recognition of its primary antigen affected its ability to be cross-reactively stimulated by MAS (a soluble product of Mycoplasma arthritidis), a panel of cloned, soluble antigen-specific I-A- and I-E-restricted T cells were tested for their ability to cross-reactivity recognize and respond to MAS. Initial studies indicated that all of the cloned T cells tested were capable of responding to MAS in the presence of genetically E.alpha.E.beta.-expressing (I-E+), but not E.alpha.E.beta.-non-expressing (I-E-) accessory cells (AC). However, subsequent studies demonstrated that the ability of most of these T cells clones to mount proliferative responses to MAS in the presence of I-E+ AC was dependent upon the presence of Lyt-1+2- T cells in the irradiated spleen cells which were used as AC sources. When T cell-depleted, I-E+ populations of spleen cells or an I-E+ antigen-presenting line (WEHI-5) were used as AC sources, only 6 of the 34 clones tested were found to be directly responsive to MAS. Subsequent to stimulation by MAS I-E product, these MAS-reactive T cell clones were capable of "recruiting" bystander T cells to proliferate. Finally, the ability of a given T cell clone to respond to MAS plus I-E product did not appear to be influenced by the restriction element used by that clone in its response to other antigens since both I-A-restricted T cell clones were responsive to MAS plus I-E in equivalent proportions. Thus, the data presented indicate that I-E-restricted T cell reactivity to MAS is a clonally expressed property of T cells that is independent of their conventional antigen specificities and MHC restriction patterns.