The role of dietary nucleotides in reduction of DNA damage induced by T-2 toxin and deoxynivalenol in chicken leukocytes
The role of dietary nucleotides in reduction of DNA damage induced by T-2 toxin and deoxynivalenol in chicken leukocytes
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DOI：
10.1016/j.fct.2006.06.002
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发表时间：
2006-11-01
影响因子：
4.3
通讯作者：
Salobir, J.
中科院分区：
文献类型：
--
作者：
Frankic, T.;Pajk, T.;Salobir, J.
本研究的目的是确定T-2毒素和脱氧雪腐镰刀菌烯醇（DON）的DNA片段化的影响。鸡脾脏白细胞和氧化应激，并进一步评估饮食核苷酸在减少毒素诱导的DNA损伤方面的潜力。雄性肉鸡暴露于10 mg/kg饲料的T-2毒素或DON，添加或不添加膳食核苷酸。治疗17天后，采用彗星试验检测脾脏白细胞DNA损伤，采用脂质过氧化反应、血浆总抗氧化状态（TAS）和谷胱甘肽过氧化物酶（GPx）测定研究脂质过氧化反应，通过测定血浆肝酶（ALT、AST和GGT）水平研究肝毒性。T-2毒素和DON均能诱导鸡脾白细胞DNA断裂，而添加核苷酸仅能降低T-2毒素对DNA的损伤。与对照组相比，在喂食T-2毒素并补充或不补充核苷酸的组中，TAS和AST值显著降低。血浆和肝脏MDA含量在组喂养T-2毒素和DON没有显着差异，从控制。当添加到含有霉菌毒素的饲料中时，饲料核苷酸不影响MDA的形成。所获得的结果表明，膳食核苷酸具有降低由T-2毒素在免疫细胞中的作用诱导的DNA损伤程度的效力。这强调了它们在霉菌毒素中毒中对免疫系统的可能有益作用。(c)2006爱思唯尔有限公司保留所有权利。
The objective of present study was to determine the effect of T-2 toxin and deoxynivalenol (DON) on DNA fragmentation in. spleen leukocytes and oxidative stress in chickens, and furthermore, to evaluate the potential of dietary nucleotides in reduction of toxin-induced DNA damage. Male broiler chickens were exposed to 10 mg/kg feed of either T-2 toxin or DON with or without addition of dietary nucleotides. After 17 days of treatment DNA damage of spleen leukocytes was measured by Comet assay, lipid peroxidation was studied by malondialdehyde (MDA), total antioxidant status (TAS) of plasma and glutathione peroxidase (GPx) assays, and the hepatotoxicity was studied by measuring plasma liver enzyme levels (ALT, AST and GGT) levels. T-2 toxin and DON induced DNA fragmentation in chicken spleen leukocytes and supplementation with nucleotides reduced the amount of damage only when added to T-2 toxin. In comparison to control group, values of TAS and AST decreased significantly in the groups fed T-2 toxin with or without nucleotide supplementation. Plasma and liver MDA content in groups fed T-2 toxin and DON did not differ significantly from the control. Dietary nucleotides did not affect MDA formation when added to the diets with mycotoxins. The results obtained suggest that dietary nucleotides have the potency to reduce the extent of DNA damage induced by the action of T-2 toxin in immune cells. This underlines their possible beneficial effect on the immune system in mycotoxin intoxication. (c) 2006 Elsevier Ltd. All rights reserved.