Capacity of "transplanted" lymphocytes to traverse the intestinal epithelium of adult rats.
Capacity of "transplanted" lymphocytes to traverse the intestinal epithelium of adult rats.
复制标题
“移植”淋巴细胞穿过成年大鼠肠上皮的能力。
DOI：
10.1097/00007890-198110000-00010
复制
发表时间：
1981
期刊：
影响因子：
6.2
通讯作者：
Billingham,RE
中科院分区：
文献类型：
--
作者：
SeeligJr,LL;Billingham,RE
将未标记和3 H-尿苷标记的成年Fischer（FI）大鼠淋巴结细胞（LNC）悬液接种于成年（FI× DA）F1杂交宿主回肠腔内。血管，淋巴管和神经供应的隔离段被保存。根据免疫遗传学特异性（归因于移植物抗宿主（GVH）反应性）和分离节段肠壁中标记淋巴样细胞的组织学鉴定，推测引流肠系膜LN复合体随后肥大将指示接种细胞穿过肠上皮。结果发现，接种10 0 × 10 6或30 0 × 10 6 FI LNC的动物，其肠系膜淋巴结在接种后第8天明显大于接种相同数量同系F1杂种细胞的动物（对照组）。这种淋巴结肥大具有剂量依赖性，并受到派伊尔集合淋巴结存在的有利影响。将3 H-尿苷标记的细胞导入离体回肠段并随后进行放射自显影的实验结果证实了沉积在肠腔中的淋巴细胞可以进入宿主组织的结论，即它们是“自然移植的”。
Suspensions of viable lymph node cells (LNCs), both unlabeled and 3 H-uridine labeled, from adult Fischer (FI) rats were inoculated into the lumens of established, surgically isolated segments of ileum in adult (FI× DA) F 1 hybrid hosts. Vascular, lymphatic, and nerve supplies of the isolated segment were preserved. It was reasoned that subsequent hypertrophy of the draining mesenteric LN complex, on an immunogenetically specific basis (attributable to graft-versus-host (GVH) reactivity) and histological identification of labeled lymphoid cells in the intestinal wall of the isolated segment, would be indicative of the passage of inoculated cells across the intestinal epithelium. It was found that host mesenteric LNs were significantly larger on the 8th postinoculation day in animals that received 100× 10 6 or 300× 10 6 FI LNCs than in animals that were given similar numbers of syngeneic F 1 hybrid cells (controls). This lymph node hypertrophy was dosage dependent and favorably influenced by the presence of Peyer's patches. The results of experiments involving introduction of 3 H-uridine-labeled cells into isolated ileal segments and subsequent radioautography corroborated the conclusion that lymphocytes deposited in the intestinal lumen can gain access to host tissues, ie, they are “naturally transplanted.”