Molecular function predictions and diagnostic value analysis of plasma exosomal miRNAs in Hirschsprung's disease
Molecular function predictions and diagnostic value analysis of plasma exosomal miRNAs in Hirschsprung's disease
复制标题
血浆外泌体miRNA对先天性巨结肠的分子功能预测及诊断价值分析
DOI：
10.2217/epi-2019-0190
复制
发表时间：
2020-03-01
期刊：
影响因子：
3.8
通讯作者：
Tang, Weibing
中科院分区：
文献类型：
--
作者：
Lv, Xiurui;Li, Yuhan;Tang, Weibing
目的：发现血浆外泌体mirna在巨结肠病（HSCR）中的潜在作用，并确定早期诊断HSCR的潜在无创生物标志物。材料与方法：收集HSCR患者和匹配对照的血浆样本。先分离外泌体，然后利用高通量Illumina测序获得失调外泌体mirna的谱，然后在两个单独的队列中进行进一步验证。我们还通过生物信息学分析来探讨巨结肠病中异常mirna的分子功能。结果与结论：共鉴定出31个异常mirna，其中5个被认为是有希望的HSCR特征。基因富集分析表明，上调的mirna最有可能参与“细胞外基质-受体相互作用”，并通过干扰细胞连接促进HSCR。
Aim: To discover the potential roles of plasma exosomal miRNAs in Hirschsprung's disease (HSCR) and identify potential noninvasive biomarkers for early diagnosis of HSCR. Materials & methods: Plasma samples were collected from HSCR patients and matched controls. Exosomes were isolated before high-throughput Illumina sequencing was utilized to gain a profile of dysregulated exosomal miRNAs, followed with further verification in two separate cohorts. Bioinformatics analyses were also adopted to explore the molecular functions of dysregulated miRNAs in Hirschsprung's disease. Results & conclusion: 31 dysregulated miRNAs were identified with five considered as promising HSCR signatures. Gene enrichment analysis disclosed that the upregulated miRNAs were most likely to participate in 'extracellular matrix-receptor interaction' and contribute to HSCR through interfering in cell junctions.