Measuring transport properties of cell membranes by a PDMS microfluidic device with controllability over changing rate of extracellular solution
Measuring transport properties of cell membranes by a PDMS microfluidic device with controllability over changing rate of extracellular solution
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DOI：
10.1016/j.snb.2014.02.045
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发表时间：
2014-07-01
影响因子：
8.4
通讯作者：
Hsieh, Wen-Hsin
中科院分区：
文献类型：
--
作者：
Lyu, Shaw-Ruey;Chen, Wun-Jie;Hsieh, Wen-Hsin
水和化学物质穿过细胞膜的运输在细胞生物学和冷冻保存中起着重要作用。在这项研究中，聚二甲基硅氧烷（PDMS）的流体动力学开关微流控装置的开发，以改变细胞外溶液在可控的速率在测量细胞膜的传输特性。为了证明PDMS微流体装置的能力，记录并分析捕获的软骨细胞对细胞外渗透压变化的体积响应，以确定水力传导率，细胞膜的运输特性之一。结果表明，液压切换是一种方便可靠的控制胞外液切换时间常数的方法。切换时间常数随着注射泵抽吸速率的增加而增加，这引起流体动力学切换。新鲜软骨细胞的两个膜的水力传导率，在两个不同的开关时间常数测量，是统计学上相同的（t检验，置信水平= 95%），这表明在这项研究中开发的方法的可信度高。本文报道的PDMS装置代表了在微流体装置中首次成功地使用流体动力学切换来控制膜特性测量中细胞外溶液的变化速率。(C)2014爱思唯尔有限公司版权所有。
The transport of water and chemicals across cell membranes plays an important role in cell biology and in cryopreservation. In this study, a polydimethylsiloxane (PDMS) microfluidic device with hydrodynamic switching is developed to change the extracellular solution at controllable rates in measuring transport properties of cell membranes. To demonstrate the capability of the PDMS microfluidic device, the volume response of trapped chondrocytes to the change in extracellular osmolality is recorded and analyzed to determine the hydraulic conductivity, one of the transport properties of cell membranes. The results show that hydraulic switching is a convenient and reliable method for controlling the switching time constant of the extracellular solution. The switching time constant increases with the increase in the aspiration rate of the syringe pump, which induces the hydrodynamic switching. The two membrane hydraulic conductivities of fresh chondrocytes, measured at two different switching time constants, are statistically identical (t-test, confidence level = 95%), which indicates a high credibility for the method developed in this study. The PDMS device reported here represents the first successful use of hydrodynamic switching in a microfluidic device to control the changing rate of extracellular solutions in the measurement of membrane properties. (C) 2014 Elsevier B.V. All rights reserved.