Cortisol modulates the induction of inflammatory gene expression in a rainbow trout macrophage cell line
Cortisol modulates the induction of inflammatory gene expression in a rainbow trout macrophage cell line
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DOI：
10.1016/j.fsi.2010.10.010
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发表时间：
2011-01-01
影响因子：
4.7
通讯作者：
Martin, Samuel A. M.
中科院分区：
文献类型：
--
作者：
Castro, Rosario;Zou, Jun;Martin, Samuel A. M.
糖皮质激素对免疫系统的作用是多样的，依赖于细胞类型，对细胞类型特异性的相互作用和激素和细胞因子之间的相互作用知之甚少。在这项研究中，我们用定量聚合酶链式反应分析了虹鲑鱼巨噬细胞系RTS-11在皮质醇和促炎细胞因子(如重组鲑鱼IL-1β，干扰素-γ)、I型干扰素或PAMP(脂多糖或PolyI：C)的组合作用下的基因表达谱。炎症过程的几个关键基因被作为目标，以评估它们的表达是否由于皮质醇的加入而发生任何调节。巨噬细胞与生理浓度的皮质醇孵育3或6h后，IL-6和IL-8的表达下降，但其他基因的表达没有明显变化。皮质醇与炎症介质的共同刺激导致了与炎症反应相关的基因的普遍抑制。共同孵育3h后，皮质醇可抑制所有刺激剂对IL-8的上调。6小时后，rIL-1β、rIFN-γ和PolyI：C对IL-6的上调、rIFN-γ或PolyI：C对γIP的上调以及rIL-1β对COX-2的上调均受到抑制。相反，皮质醇与促炎剂联合对IL-10的表达具有协同作用，提示在皮质醇处理后，某些巨噬细胞功能的激活导致炎症的缓解。(C)2010爱思唯尔有限公司。保留所有权利。
Glucocorticoid actions on the immune system are diverse and cell type dependent, and little is known about cell type-specific interactions and cross-talk between hormones and cytokines. In this study we have analyzed the gene expression patterns of the rainbow trout macrophage cell line RTS-11 by quantitative PCR, after exposure to combinations of cortisol plus a pro-inflammatory cytokine (e.g. recombinant trout IL-1 beta, IFN-gamma), type I IFN or a PAMP (LPS or poly I:C). Several key genes of the inflammatory process were targetted to assess whether any modulation of their expression occurred due to the addition of cortisol to this cell line. Incubation of macrophages for 3 or 6 h with a physiological concentration of cortisol caused a decrease in expression of IL-6 and IL-8, but no significant changes were observed for the other genes examined. Co-stimulation of cortisol with the inflammatory agents resulted in a general suppression of genes related to the inflammatory response. Cortisol inhibited the up-regulation of IL-8 by all the stimulants after 3 h of co-incubation. Suppression of the up-regulation of IL-6 by rIL-1 beta, rIFN-gamma and poly I:C, of gamma IP by rIFN-gamma or poly I:C, and of Cox-2 by rIL-1 beta was seen after 6 h. In contrast, cortisol in combination with the pro-inflammatory agents has a synergistic effect on IL-10 expression, an anti-inflammatory molecule, suggesting that the activation of certain macrophage functions that lead to the resolution of inflammation occurs in fish macrophages in response to cortisol treatment. (C) 2010 Elsevier Ltd. All rights reserved.