In vitro antibacterial activity and cytocompatibility of bismuth doped micro-arc oxidized titanium
In vitro antibacterial activity and cytocompatibility of bismuth doped micro-arc oxidized titanium
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DOI：
10.1177/0885328211414942
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发表时间：
2013-01-01
影响因子：
2.9
通讯作者：
Fuh, Lih-Jyh
中科院分区：
文献类型：
--
作者：
Lin, Dan-Jae;Tsai, Ming-Tzu;Fuh, Lih-Jyh
种植体表面的化学处理产生杀菌特征，以防止牙科种植体周围的感染。尽管铋已成功用于粘膜和真皮感染，但铋在口腔中的抗菌作用仍在研究中。本研究的目的是评价铋化合物对伴放线杆菌、变形葡萄球菌和耐甲氧西林金黄色葡萄球菌（MRSA）的抗菌活性，并通过琼脂扩散试验研究掺铋微弧氧化（MAO）钛的抗菌效果。在第1、7和14天评价接种在涂层上的MG 63成骨细胞样细胞的细胞活力、碱性磷酸酶活性和矿化水平。结果表明，与醋酸铋、碱式没食子酸铋和硝酸银相比，硝酸铋具有上级的抗菌活性。铋掺杂的微弧氧化膜（含6.2原子百分比的铋）对MG 63细胞具有良好的生物亲和性，对伴放线杆菌和MRSA具有较高的抗菌效果，菌落数减少率分别比对照组高1.5倍和1.9倍。这些体外评价表明，表面含铋的钛植入物可能有助于更好地控制感染。
Chemical manipulations of the implant surface produce a bactericidal feature to prevent infections around dental implants. Despite the successful use of bismuth against mucosal and dermis infections, the antibacterial effect of bismuth in the oral cavity remains under investigation. The aim of this study was to evaluate the antibacterial activities of bismuth compounds against Actinobacillus actinomycetemcomitans, Staphylococcus mutans, and methicillin-resistant Staphylococcus aureus (MRSA), and to investigate the antimicrobial effects of bismuth doped micro-arc oxidation (MAO) titanium via an agar diffusion test. Cell viability, alkaline phosphatase activity, and mineralization level of MG63 osteoblast-like cells seeded on the coatings were evaluated at 1, 7, and 14 days. The results demonstrate that bismuth nitrate possess superior antibacterial activity when compared with bismuth acetate, bismuth subgallate, and silver nitrate. The bismuth doped MAO coating (contained 6.2 atomic percentage bismuth) had good biological affinities to the MG63 cells and showed a higher antibacterial efficacy against Actinobacillus actinomycetemcomitans and MRSA, where the reduction rates of colony numbers is higher than that of the control group by 1.5 and 1.9 times, respectively. These in vitro evaluations demonstrate that titanium implants with bismuth on the surface may be useful for better infection control.