Identification and characterization of microRNAs and their targets from expression sequence tags of Ribes nigrum
Identification and characterization of microRNAs and their targets from expression sequence tags of Ribes nigrum
复制标题
黑醋栗表达序列标签中 microRNA 及其靶标的鉴定和表征
DOI：
10.1139/cjps-2016-0013
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发表时间：
2016-04
影响因子：
1.2
通讯作者：
Bian Shaomin
中科院分区：
文献类型：
--
作者：
Xie Xin;Li Xuyan;Tian Youxia;Su Mojie;Zhang Jiayan;Han Xue;Cui Yuhai;Bian Shaomin
摘要：MicroRNAs （miRNAs）是一类内源性的单链非编码rna，长度约为21 nt，在植物的生物学和代谢过程中起着关键作用。虽然已经在许多植物物种中发现了许多mirna，但在Ribes nigrum中仍然完全未知。在这项研究中，使用基于表达序列标签（EST）的比较基因组学方法首次在Ribes nigrum中鉴定出两个miRNAs （rni-miR5021和rni-miR5185）。随后，转基因分析表明，这些推测的MIR基因编码rni-miR5021和rni-miR5185能够在体内产生相应的miRNA。此外，定量反转录聚合酶链反应（qRT-PCR）分析表明，MIR基因在黑加仑各组织中的表达模式存在差异，两种成熟mirna在果实中的积累量高于其他组织。最后，我们预测了这两种mirna的47个靶点，它们参与了对胁迫和其他植物生物过程的响应。这些研究结果将为今后对里氏线虫中mirna的功能和调控机制的研究提供参考。
Abstract: MicroRNAs (miRNAs) are a class of endogenous, single-stranded, approximately 21 nt in length, non-coding RNAs which play critical roles in plant biological and metabolic processes. Although numerous miRNAs have been identified in many plant species, miRNAs still remain totally unknown in Ribes nigrum. In the study, two miRNAs (rni-miR5021 and rni-miR5185) were firstly identified in Ribes nigrum using an expression sequence tag (EST)-based comparative genomics approach. Subsequently, transgenic analysis suggested that these putative MIR genes encoding rni-miR5021 and rni-miR5185 were able to generate their corresponding miRNA in vivo. Furthermore, quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis indicated that the expression patterns of the MIR genes varied among blackcurrant tissues, and the two mature miRNAs showed higher accumulation in fruits than other tissues. Finally, 47 targets were predicted for the two miRNAs, which are involved in response to stresses and other plant biological processes. These findings will facilitate future studies on the functions and regulatory mechanisms of miRNAs in Ribes nigrum.
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影响因子：
4.4
作者：
Thakur V;Wanchana S;Xu M;Bruskiewich R;Quick WP;Mosig A;Zhu XG
通讯作者：
Zhu XG
DOI：
10.1111/j.1365-2141.1970.tb00770.x
发表时间：
1935-04
期刊：
Glasgow Medical Journal
影响因子：
--
作者：
W. Pitney
通讯作者：
W. Pitney
影响因子：
5.8
作者：
Bonnet, E;Wuyts, J;Van de Peer, Y
通讯作者：
Van de Peer, Y
影响因子：
--
作者：
Penny A C Sparrow;Philip J Dale;J. Irwin
通讯作者：
Penny A C Sparrow;Philip J Dale;J. Irwin
影响因子：
7.2
作者：
Zhang, BH;Pan, XP;Anderson, TA
通讯作者：
Anderson, TA