Long-term serological evaluation of patients with cystic echinococcosis treated with benzimidazole carbamates
Long-term serological evaluation of patients with cystic echinococcosis treated with benzimidazole carbamates
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DOI：
10.1046/j.1365-2249.2002.01925.x
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发表时间：
2002-09-01
影响因子：
4.6
通讯作者：
Siracusano, A
中科院分区：
文献类型：
--
作者：
Riganò, R;Ioppolo, S;Siracusano, A
为了寻找更好的免疫学标志物，表明囊型包虫病（CE）化疗后的长期结果，我们研究了23例接受阿苯达唑的患者，临床随访8年，并根据治疗结果进行超声分组。通过间接血凝（IHA）、ELISA和免疫印迹（IB）评价抗部分纯化的包虫液（HFF）和抗原B（Ag B）的抗体应答。虽然IHA滴度在治疗过程中变化，但组间HFF平均抗体滴度的差异仅在4年时具有显著性（P = 0.031）。IgG同种型表达在随访期间保持不变，而IgE表达在治愈或疾病稳定的患者中下降。AgB组IgG 4表达高于HFF组（P < 10（-4）; P = 0.025），IgG 1表达低于HFF组（P < 10（-4）; P = 0.022）。IHA抗体滴度在进行性患者中高于治愈或稳定的患者，即使在相同的囊肿类型中。两组间的ELISA同种型谱不同，特别是CE 3、4和5型囊肿：治愈或稳定疾病患者的血清IgG 1和IgG 3较高，IgG 4和IgE较低。虽然联合血清学检测提供的信息很少CE化疗后的长期结果，它可能是有用的回顾性研究的结果囊肿和评估宿主-寄生虫的关系。
Seeking better immunological markers indicating the long-term outcome of cystic echinococcosis (CE) after chemotherapy we studied 23 patients receiving albendazole, clinically followed for 8 years, and grouped ultrasonographically according to therapeutic outcome. Antibody responses against a partially purified fraction of hydatid fluid (HFF) and antigen B (AgB) were evaluated by indirect haemagglutination (IHA), ELISA and immunoblotting (IB). Although IHA titres varied over the course of treatment, differences in mean antibody titres to HFF between groups were significant only at 4 years (P = 0.031). IgG isotype expression remained unchanged during follow-up whereas IgE expression decreased in patients with cured or stable disease. AgB disclosed higher IgG4 expression (P < 10(-4) ; P = 0.025) and lower IgG1 expression than HFF (P < 10(-4) ; P = 0.022). IHA antibody titres were higher in patients with progressive than in those with cured or stable disease, even in those with the same cyst type. ELISA isotype profiles differed between groups, particularly for type CE 3, 4 and 5 cysts: higher serum IgG1 and IgG3, lower IgG4 and IgE in patients with cured or stable disease. Although combined serological testing provides scarce information on the long-term outcome of CE after chemotherapy it may be useful for reviewing in a retrospective study the outcome of a cyst and for assessing the host-parasite relationship.