Epidermal growth factor from the mouse. Physical evidence for a tiered beta-sheet domain: two-dimensional NMR correlated spectroscopy and nuclear Overhauser experiments on backbone amide protons.
Epidermal growth factor from the mouse. Physical evidence for a tiered beta-sheet domain: two-dimensional NMR correlated spectroscopy and nuclear Overhauser experiments on backbone amide protons.
复制标题
来自小鼠的表皮生长因子。
DOI：
10.1021/bi00335a055
复制
发表时间：
1985
期刊：
影响因子：
2.9
通讯作者：
Mayo,KH
中科院分区：
文献类型：
--
作者：
Mayo,KH
修订稿于 1984 年 12 月 27 日收到 摘要：当 H2O 交换的冻干小鼠表皮生长因子 (mEGF) 在低 pH 值（即低于 6.0）下溶解在氧化氘中时，在 NMR 谱（500 MHz）的低场区域观察到 13 个分辨率良好的酰胺质子共振。在这些实验的条件下，这些酰胺质子与氧化氘溶剂的氘核交换的寿命表明这些酰胺质子是氢键键合的主链酰胺质子。这些酰胺质子共振中的一些显示出约8-10 Hz的分裂（即/nho-ch），表明它们相关的酰胺质子处于某种类型的0-结构中。对所有酰胺质子共振进行的选择性核奥弗豪瑟效应 (NOE) 实验强烈表明，所有 13 个主链酰胺质子都是 mEGF 中单层 0 片结构域的一部分。 2D NMR 相关光谱数据的关联，识别标度耦合质子，与 NOE 数据，识别靠近辐照酰胺质子的质子，允许将 NOE 差异光谱中的一些共振暂时分配给特定的氨基酸残基。这些数据允许假设 mEGF 中分层 0-sheet 结构域的部分结构模型。蛋白质激素，小鼠表皮生长因子 (mEGF)，1 刺激各种表皮和上皮组织的生长和分化 (Cohen, 1962, 1965; Cohen & Elliott, 1963; Turkington, 1969; Savage & Cohen, 1973)。在细胞水平上，mEGF 通过与跨膜受体分子结合并成为 f 直接作用于皮肤细胞。这项工作得到了美国国立卫生研究院 (GM-34662) 的资助和 Glenmede 信托基金的慷慨捐赠，并受益于通过国家科学基金会的 CHE-7916210 资助向耶鲁大学提供的 NMR 设施。
Revised Manuscript Received December 27, 1984 abstract: When H20-exchanged, lyophilized mouse epidermal growth factor (mEGF) is dissolved in deuterium oxideat low pH (ie, below~ 6.0), 13 well-resolved, amide proton resonances are observed in the downfield region of an NMR spectrum (500 MHz). Under the conditions of these experiments, the lifetimes of these amide protons in exchange for deuterons of the deuterium oxide solvent suggest that these amide protons are hydrogen-bonded, backbone amide protons. Several of these amide proton resonances show splittings (ie,/nho-ch) f approximately 8-10 Hz, indicating that their associated amide protons are in some type of 0-structure. Selective nuclear Overhauser effect (NOE) experiments performed on all amide proton resonances strongly suggest that all 13 of these backbone amide protons are part of a single-tiered 0-sheet structural domain in mEGF. Correlation of 2D NMR correlated spectroscopy data, identifying scaler coupled protons, with NOE data, identifying protons close to the irradiated amide protons, allows tentative assignment of some resonances in the NOE difference spectra to specific amino acid residues. These data allow a partial structural model of the tiered 0-sheet domain in mEGF to be postulated. e protein hormone, mouse epidermal growth factor (mEGF), 1 stimulates the growth and differentiation of various epidermal and epithelial tissues (Cohen, 1962, 1965; Cohen & Elliott, 1963; Turkington, 1969; Savage & Cohen, 1973). On a cellular level, mEGF works directly on skin cells by binding to a transmembrane receptor molecule and becoming f This work was supported by a grant from the National Institutes of Health (GM-34662) and by a generous gift from the Glenmede Trust Fund and benefited from NMR facilities made available to YaleUniversity through Grant CHE-7916210 from the National Science Foundation.