IDENTIFICATION OF PRE-SYNAPTIC NEURONS BY LASER PHOTOSTIMULATION
IDENTIFICATION OF PRE-SYNAPTIC NEURONS BY LASER PHOTOSTIMULATION
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DOI：
10.1126/science.6648515
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发表时间：
1983-01-01
期刊：
影响因子：
56.9
通讯作者：
GRINVALD, A
中科院分区：
文献类型：
--
作者：
FARBER, IC;GRINVALD, A
开发了一种光学方法，涉及使用激光和新型荧光染料作为光刺激探针，以识别大量细胞[藤壶、青蛙、水蛭、海兔]中的突触前神经元。用激光微束照射细胞外染色的神经元可诱发动作电位。利用这种技术，连接识别水蛭神经元的中间神经元被迅速定位。该方法加快了神经元网络的阐明，特别是当涉及小细胞时。
An optical method involving the use of a laser and a novel fluorescent dye as a photostimulation probe was developed to identify presynaptic neurons in a large ensemble of cells [barnacle, frog, leech, Aplysia]. Illumination of an extracellularly stained neuron by the laser microbeam evokes action potentials. With this technique an interneuron connecting identified leech neurons was quickly located. The method speeds up the elucidation of neuronal networks, especially when small cells are involved.