The effects of osteoporosis and disc degeneration on vertebral cartilage endplate lesions in rats
The effects of osteoporosis and disc degeneration on vertebral cartilage endplate lesions in rats
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DOI：
10.1007/s00586-014-3324-9
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发表时间：
2014-09-01
影响因子：
2.8
通讯作者：
Zhu, Qingan
中科院分区：
文献类型：
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作者：
Ding, Yin;Jiang, Jie;Zhu, Qingan
有证据表明，骨质疏松症或椎间盘退变（IDD）会导致软骨终板病变（CEL），但它们对病变的综合影响仍不清楚。本研究开发了一种创新的卵巢切除术（OVX）和颈肌切片（CMS）联合大鼠模型，旨在评估骨质疏松症和IDD对颈椎软骨终板病变的联合影响。 将52只Sprague-Dawley雌性大鼠随机分为四组：假手术组（n = 10）进行假手术； OVX组（n = 14）接受双侧卵巢切除术； CMS 组（n = 14）从 C2 到 C7 切除后椎旁肌； CMS-OVX组（n = 14）连续接受OVX和CMS手术。术后 12、18 和 24 周采集 C6-C7 节段样本。进行显微 CT 分析以评估 CEL、椎间盘高度 (IDH) 和结构指数。采用番红O/固绿染色组织学分析和组织学评分观察退变椎间盘的特征。卵巢切除手术导致C6体大部分结构指标发生显着变化，如12周时骨体积百分比和骨小梁数量下降，18周和24周时变化更大。 CMS 手术后的 CEL 见于腹侧，而 OVX 组和假手术组的 CEL 见于外周。 CMS-OVX 组的 CEL 最大，并且在 12 周和 18 周时显着高于 CMS 和 OVX 组（P < 0.05）。与假手术组相比，CMS 手术导致 IDH 在 12、18 和 24 周显着下降（P < 0.05），而 OVX 手术导致 IDH 轻度下降。 24周时CMS-OVX组IDH显着低于CMS组（P < 0.05）。组织学评估表明，CMS 和 CMS-OVX 组在 12 周时出现软骨终板磨损，在 18 周和 24 周时出现原位钙化。 CMS或OVX手术后椎间盘退变评分较高，并分别与CEL和IDH相关（P < 0.01）。本研究表明，OVX和CMS组合比其中任何一种手术导致更多的软骨终板损伤，以及更多的IDH下降。病变和 IDH 降低与椎间盘退变水平相关。软骨终板早期磨损，后期原位钙化。结果表明，骨质疏松症可能会在特定时间加剧椎间盘退变。
Evidence has shown that osteoporosis or intervertebral disc degeneration (IDD) led to cartilage endplate lesions (CEL), but their combined effects on the lesion remain unknown. This study developed an innovative rat model combined ovariectomy (OVX) and cervical muscle section (CMS), and aimed to evaluate the combined effects of osteoporosis and IDD on cartilage endplate lesions of cervical spine.Fifty-two Sprague-Dawley female rats were assigned randomly into four groups as follows: the sham group (n = 10) underwent sham surgery; the OVX group (n = 14) was subjected to bilateral ovariectomy; the CMS group (n = 14) had posterior paraspinal muscles cut from C2 to C7; the CMS-OVX group (n = 14) underwent the OVX and CMS surgeries consecutively. Samples of C6-C7 segments were harvested at 12, 18 and 24 weeks post-surgery. Micro-CT analysis was performed to evaluate the CEL, intervertebral disc height (IDH) and structural indices. Histological analysis with Safranine O/fast green stain and histological score were used to observe the characteristics of the degenerative discs.Ovariectomy surgery resulted in significant changes of most structural indices of the C6 body, such as decrease of percent bone volume and number of bone trabecula at 12 weeks, and greater changes at 18 and 24 weeks. The CEL following CMS surgery was seen on the ventral, while the CEL in the OVX and sham groups on the peripheral. The CEL was greatest in the CMS-OVX group and significantly greater than that in the CMS and OVX groups at 12 and 18 weeks (P < 0.05). The CMS surgery resulted in significant IDH decrease at 12, 18 and 24 weeks (P < 0.05), while the OVX surgery resulted in mild IDH decrease when compared with the sham group. The IDH in the CMS-OVX group was significantly lower than that in the CMS group at 24 weeks (P < 0.05). Histological evaluation suggested cartilage endplate abrasion at 12 weeks, and in situ calcification at 18 and 24 weeks in the CMS and CMS-OVX groups. Disc degenerative scores were higher following CMS or OVX surgery, and correlated with the CEL and IDH (P < 0.01), respectively.The present study suggested that a combination of OVX and CMS led to more lesion of cartilage endplate than any one thereof, as well as more decrease of IDH. The lesion and IDH decrease were associated with the disc degeneration levels. The cartilage endplate was worn out at the early stage and calcified in situ later. The results indicate that osteoporosis may deteriorate the disc degeneration at specific time.