Non-structural protein 1 (NS1) antibody-based assays to differentiate West Nile (WN) virus from Japanese encephalitis virus infections in horses: Effects of WN virus NS1 antibodies induced by inactivated WN vaccine
Non-structural protein 1 (NS1) antibody-based assays to differentiate West Nile (WN) virus from Japanese encephalitis virus infections in horses: Effects of WN virus NS1 antibodies induced by inactivated WN vaccine
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DOI：
10.1016/j.jviromet.2010.10.012
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发表时间：
2011-01-01
影响因子：
3.1
通讯作者：
Konishi, Eiji
中科院分区：
文献类型：
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作者：
Kitai, Yoko;Kondo, Takashi;Konishi, Eiji
针对西尼罗河病毒（WNV）的非结构蛋白1（NS1）的抗体已被用于区分马中的WNV感染与血清学交叉反应黄病毒（包括日本脑炎病毒（JEV））感染。然而，由于灭活西尼罗河（WN）疫苗已报告可诱导NS1抗体，因此人们担心使用基于NS1的检测方法检测接种疫苗的马的可靠性。因此，研究了灭活WN疫苗诱导的抗体对表位阻断ELISA和补体依赖性细胞毒性（CDC）测定的影响。这两种检测方法都是基于NS1抗体，并且先前建立用于区分马中的WNV和JEV感染。每组三匹马接种两剂或三剂商业灭活WN疫苗，第二次接种后通过常规ELISA检测NS1抗体。疫苗诱导的NS1抗体也通过阻断ELISA和CDC试验检测到，并影响这些试验区分WNV和JEV感染的能力。然而，在CDC测定中，该效应不太显著，其中使用低血清浓度确保了有效的分化。通过CDC测定法对感染诱导的抗体的检测效率高于疫苗诱导的抗体，这可能归因于这些抗体的不同IgG同种型谱。(C)2010 Elsevier B.V.保留所有权利。
Antibodies to non-structural protein 1 (NS1) of West Nile virus (WNV) have been used to differentiate WNV infection from infection by serologically cross-reactive flaviviruses, including Japanese encephalitis virus (JEV), in horses. However, since the inactivated West Nile (WN) vaccine has been reported to induce NS1 antibodies, there is concern about the reliability of using NS1-based assays for testing vaccinated horses. Therefore, the effect of inactivated WN vaccine-induced antibodies on an epitope-blocking ELISA and complement-dependent cytotoxicity (CDC) assay were investigated. Both assays are based on NS1 antibodies and were established previously to differentiate WNV from JEV infections in horses. Groups of three horses were vaccinated with two or three doses of a commercial inactivated WN vaccine and NS1 antibodies were detected by a conventional ELISA after the second vaccination. Vaccine-induced NS1 antibodies were also detected by blocking ELISA and a CDC assay and affected the ability of these assays to differentiate WNV from JEV infections. However, the effect was less significant in the CDC assay, where use of a low serum concentration ensured effective differentiation. The more efficient detection of infection-induced antibodies over vaccine-induced antibodies by the CDC assay was potentially attributable to the different IgG isotype profiles of these antibodies. (C) 2010 Elsevier B.V. All rights reserved.