Comparison of In Vitro Inactivation of SARS CoV-2 with Hydrogen Peroxide and Povidone-Iodine Oral Antiseptic Rinses
Comparison of In Vitro Inactivation of SARS CoV-2 with Hydrogen Peroxide and Povidone-Iodine Oral Antiseptic Rinses
复制标题
DOI：
10.1111/jopr.13220
复制
发表时间：
2020-07-24
影响因子：
4
通讯作者：
Tessema, Belachew
中科院分区：
文献类型：
--
作者：
Bidra, Avinash S.;Pelletier, Jesse S.;Tessema, Belachew
目的 评估在临床推荐浓度和接触时间下过氧化氢 (H2O2) 和聚维酮碘 (PVP-I) 口腔消毒漱口液对严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2) 的体外灭活效果。材料和方法 SARS-CoV-2、USA-WA1/2020 株病毒储备液在测试前通过在 Vero 76 细胞中生长来制备。制备病毒原液的培养基是含有2%胎牛血清（FBS）和50μg/mL庆大霉素的最低必需培养基（MEM）。将由PVP-I口腔冲洗液和H(2)O(2)水溶液组成的测试化合物直接与病毒溶液混合，使得最终浓度为测试化合物的50％和病毒溶液的50％。因此，PVP-I的测试浓度为0.5%、1.25%和1.5%，H(2)O(2)的测试浓度为3%和1.5%，以代表临床推荐的浓度。乙醇和水作为标准阳性和阴性对照进行平行评估。所有样品均在 15 秒和 30 秒的接触时间下进行测试。然后通过标准终点稀释测定对每个样品中的存活病毒进行定量，并计算每种化合物与阴性对照相比的对数减少值。结果 在 15 秒和 30 秒的接触时间后，0.5%、1.25% 和 1.5% 3 个浓度的 PVP-I 口腔抗菌冲洗液完全灭活了 SARS-CoV-2。浓度为 1.5% 和 3.0% 的 H(2)O(2) 溶液在接触时间 15 秒和 30 秒后显示出最小的杀病毒活性。结论 在体外，最低浓度 0.5%、最短接触时间 15 秒，PVP-I 口腔消毒漱口水可完全灭活 SARS-CoV-2 病毒。推荐的口腔冲洗液浓度为 1.5% 和 3.0% 的过氧化氢在接触时间长达 30 秒后，作为杀病毒剂的效果微乎其微。因此，在 COVID-19 大流行期间，使用 0.5% 至 1.5% 范围内的稀释 PVP-I 进行术前冲洗可能优于过氧化氢。
Purpose To evaluate the in vitro inactivation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) with hydrogen peroxide (H2O2) and povidone-iodine (PVP-I) oral antiseptic rinses at clinically recommended concentrations and contact times. Materials and Methods SARS-CoV-2, USA-WA1/2020 strain virus stock was prepared prior to testing by growing in Vero 76 cells. The culture media for prepared virus stock was minimum essential medium (MEM) with 2% fetal bovine serum (FBS) and 50 mu g/mL gentamicin. Test compounds consisting of PVP-I oral rinse solutions and H(2)O(2)aqueous solutions were mixed directly with the virus solution so that the final concentration was 50% of the test compound and 50% of the virus solution. Thus PVP-I was tested at concentrations of 0.5%, 1.25%, and 1.5%, and H(2)O(2)was tested at 3% and 1.5% concentrations to represent clinically recommended concentrations. Ethanol and water were evaluated in parallel as standard positive and negative controls. All samples were tested at contact periods of 15 seconds and 30 seconds. Surviving virus from each sample was then quantified by standard end-point dilution assay and the log reduction value of each compound compared to the negative control was calculated. Results After the 15-second and 30-second contact times, PVP-I oral antiseptic rinse at all 3 concentrations of 0.5%, 1.25%, and 1.5% completely inactivated SARS-CoV-2. The H(2)O(2)solutions at concentrations of 1.5% and 3.0% showed minimal viricidal activity after 15 seconds and 30 seconds of contact time. Conclusions SARS-CoV-2 virus was completely inactivated by PVP-I oral antiseptic rinse in vitro, at the lowest concentration of 0.5 % and at the lowest contact time of 15 seconds. Hydrogen peroxide at the recommended oral rinse concentrations of 1.5% and 3.0% was minimally effective as a viricidal agent after contact times as long as 30 seconds. Therefore, preprocedural rinsing with diluted PVP-I in the range of 0.5% to 1.5% may be preferred over hydrogen peroxide during the COVID-19 pandemic.