Determination of molecular species of calcium salts of MDP produced through decalcification of enamel and dentin by MDP-based one-step adhesive
Determination of molecular species of calcium salts of MDP produced through decalcification of enamel and dentin by MDP-based one-step adhesive
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DOI：
10.4012/dmj.2014-260
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发表时间：
2015-04-01
影响因子：
2.5
通讯作者：
Nishiyama, Norihiro
中科院分区：
文献类型：
--
作者：
Yokota, Yoko;Nishiyama, Norihiro
将牙釉质和牙本质颗粒加入到实验性的基于10-甲基丙烯酰氧基癸基磷酸二氢酯（MDP）的一步粘合剂中以反应30 s。在使用X射线衍射（XRD）和磷-31核磁共振（P-31 NMR）技术分析釉质和牙本质反应物之后，对釉质和牙本质反应物的P-31 NMR谱进行曲线拟合分析。通过改变氯化钙与MDP的摩尔比，合成了一系列三种类型的MDP钙盐。通过XRD和P-31 NMR分析，确定了牙釉质或牙本质脱钙生成的MDP钙盐（MDP-Ca盐）的分子形态。曲线拟合分析表明，釉质和牙本质在脱钙过程中形成了多种类型的MDP-Ca盐和无定形磷酸氢钙二水合物（DCPD）。牙釉质和牙本质产生的MDP钙盐的分子形式为MDP单体和MDP二聚体的单钙盐。此外，牙本质产生MDP二聚体的二钙。
Enamel and dentin particles were added to an experimental 10-methacryloyloxydecyl dihydrogen phosphate (MDP)-based one-step adhesive to react for 30 s. After enamel and dentin reactants were analyzed using X-ray diffraction (XRD) and phosphorus-31 nuclear magnetic resonance (P-31 NMR) techniques, curve-fitting analysis was performed on the P-31 NMR spectra of enamel and dentin reactants. By varying the molar ratio of calcium chloride to MDP, a series of three types of MDP-Ca salts were synthesized. The molecular species of calcium salts of MDP (MDP-Ca salts) produced by decalcification of enamel or dentin were determined based on the XRD and P-31 NMR analysis results of these three types of synthesized MDP-Ca salts. Curve-fitting analysis showed that enamel and dentin developed several types of MDP-Ca salts and amorphous dicalcium phosphate dihydrate (DCPD) during decalcification. The molecular species of MDP-Ca salts produced by enamel and dentin were mono-calcium salts of MDP monomer and MDP dimer. In addition, dentin produced a di-calcium of MDP dimer.