Phosphorylated GSK-3β protects stress-induced apoptosis of myoblasts via the PI3K/Akt signaling pathway
Phosphorylated GSK-3β protects stress-induced apoptosis of myoblasts via the PI3K/Akt signaling pathway
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磷酸化 GSK-3β 通过 PI3K/Akt 信号通路保护应激诱导的成肌细胞凋亡
DOI：
10.3892/mmr.2020.11105
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发表时间：
2020-04
影响因子：
3.4
通讯作者：
Xiao Yuan
中科院分区：
文献类型：
--
作者：
Meixi Liu;Xia Huang;Yihong Tian;Xiao Yan;Fang Wang;Junbo Chen;Qi Zhang;Qiang Zhang;Xiao Yuan
面颌肌参与颌面部畸形的发生、发展、治疗和维持。该组织的结构和功能可以通过外部刺激的变化而改变，并且整形外科医生可以使用矫形力来调节其重建。PI 3 K/Akt信号通路在细胞增殖、存活和凋亡中的生物学功能最为人们所熟知。本研究探讨PI 3 K/Akt信号通路在周期性牵张诱导的成肌细胞凋亡中的作用。为此，L 6大鼠成肌细胞培养下的机械刺激和处理的PI 3 K激酶抑制剂，LY 294002，以阐明PI 3 K/Akt信号通路的作用。Hoechst 33258染色观察成肌细胞形态学变化和凋亡情况，Western blotting检测Akt、磷酸化（p）-Akt（Ser 473）、糖原合成酶激酶3β（GSK-3β）和p-GSK-3β（Ser 9）的表达。加入PI 3 K抑制剂后，总Akt和GSK-3β的表达在各组间无显著差异;但与单独负荷应激组相比，加药组p-Akt和p-GSK-3β的水平较低。此外，周期性牵张后成肌细胞凋亡率呈时间依赖性增加，在24 h达到峰值。总之，还证明了PI 3 K/Akt/GSK-3β通路在牵张诱导的成肌细胞凋亡中起重要作用。
Facial jaw muscle is involved in the occurrence, development, treatment and maintenance of maxillofacial deformities. The structure and function of this tissue can be altered by changes in external stimuli, and orthodontists can regulate its reconstruction using orthopedic forces. The PI3K/Akt signaling pathway is most well-known for its biological functions in cell proliferation, survival and apoptosis. In the present study, the effects of the PI3K/Akt signaling pathway in cyclic stretch-induced myoblast apoptosis were investigated. For this purpose, L6 rat myoblasts were cultured under mechanical stimulation and treated with the PI3K kinase inhibitor, LY294002, to elucidate the role of the PI3K/Akt signaling pathway. Cells were stained with Hoechst 33258 to visualize morphological changes and apoptosis of myoblasts, and western blotting was performed to detect expression of Akt, phosphorylated (p)-Akt (Ser473), glycogen synthase kinase 3β (GSK-3β) and p-GSK-3β (Ser9). After addition of PI3K inhibitor, the expression of total Akt and GSK-3β did not significantly differ among groups; however, the levels of p-Akt and p-GSK-3β were lower in inhibitor-treated groups than in those treated with loading stress alone. In addition, the rate of apoptosis in myoblasts subjected to cyclic stretch increased in a time-dependent manner, peaking at 24 h. Collectively, it was also demonstrated that the PI3K/Akt/GSK-3β pathway plays an important role in stretch-induced myoblast apoptosis.
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影响因子：
8.8
作者：
Bian-hua Zhou;Pan-pan Tan;L. Jia;Wen-peng Zhao;Ji-cang Wang;Hong-wei Wang
通讯作者：
Bian-hua Zhou;Pan-pan Tan;L. Jia;Wen-peng Zhao;Ji-cang Wang;Hong-wei Wang
隐丹参酮通过 JAK2/STAT3 和 PI3K/Akt/NF kappa B 通路诱导胆管癌细胞的细胞周期停滞和细胞凋亡
DOI：
10.2147/dddt.s132488
发表时间：
2017
期刊：
Drug design, development and therapy
影响因子：
--
作者：
Ke F;Wang Z;Song X;Ma Q;Hu Y;Jiang L;Zhang Y;Liu Y;Zhang Y;Gong W
通讯作者：
Gong W
影响因子：
37.8
作者：
Cai, ZQ;Semenza, GL
通讯作者：
Semenza, GL
影响因子：
2.8
作者：
Yang, Jian;Chen, Lihua;Li, Xinxin
通讯作者：
Li, Xinxin
影响因子：
2.6
作者：
DiBiase, A. T.;Cobourne, M. T.;Lee, R. T.
通讯作者：
Lee, R. T.