Dengue Plaque Reduction Neutralization Test (PRNT) in Primary and Secondary Dengue Virus Infections: How Alterations in Assay Conditions Impact Performance
Dengue Plaque Reduction Neutralization Test (PRNT) in Primary and Secondary Dengue Virus Infections: How Alterations in Assay Conditions Impact Performance
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DOI：
10.4269/ajtmh.2009.08-0625
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发表时间：
2009-11-01
影响因子：
3.3
通讯作者：
Endy, Timothy P.
中科院分区：
文献类型：
--
作者：
Thomas, Stephen J.;Nisalak, Ananda;Endy, Timothy P.
登革热病毒（DENV）感染是一个日益恶化的全球健康问题。空斑减少中和试验 (PRNT) 目前被认为是表征和量化抗 DENV 中和抗体 (NAb) 循环水平的“金标准”。目前正在使用 PRNT 的许多变体，并且实验室之间的测定及其性能条件都尚未标准化或协调。我们使用来自经历原发性和继发性 DENV 感染的儿童的一组经过充分表征的急性期和晚期恢复期随访血清样本来评估登革热 PRNT 在各种测试条件下的性能。研究人员在同一样本组的多次检测运行中改变细胞类型、控制病毒传代以及补体的使用。我们的研究结果表明，随着不同的测试条件，PRNT 滴度结果存在很大差异。
Dengue virus (DENV) infection is a worsening global health problem. The plaque reduction neutralization test (PRNT) is currently considered to be the "gold standard" to characterize and quantify circulating levels of anti-DENV neutralizing antibody (NAb). Many variations of the PRNT are currently in use and neither the assay nor its performance conditions have been standardized or harmonized between laboratories. We used a well-characterized panel of acute and late convalescent follow-up sera samples from children experiencing primary and secondary DENV infections to evaluate the performance of the dengue PRNT under a variety of testing conditions. Investigators varied cell type, control virus passage, and the use of complement across multiple assay runs of the same sample panel. Our findings indicate wide variation in PRNT titer results in response to varied testing conditions.