ELECTRICAL CONNEXIONS BETWEEN HORIZONTAL CELLS IN DOGFISH RETINA
ELECTRICAL CONNEXIONS BETWEEN HORIZONTAL CELLS IN DOGFISH RETINA
复制标题
DOI：
10.1113/jphysiol.1971.sp009370
复制
发表时间：
1971-01-01
影响因子：
5.5
通讯作者：
KANEKO, A
中科院分区：
文献类型：
--
作者：
KANEKO, A
1.在角鲨中，研究了产生S电位的细胞对之间的电连接。记录后，通过荧光显微镜下注射两种不同的荧光染料，从形态学上确定两种细胞的类型和位置.在外部和内部水平细胞中观察到超极化S电位;在双极细胞中观察到去极化反应。检查的所有外部水平细胞对都是电耦合的，即一个水平细胞的细胞内极化引起其相邻细胞的极化。检测到与其他五个细胞分离的细胞偶联。电气耦合为非整流。外水平细胞与内水平细胞之间、外水平细胞与双极细胞之间均未发现耦合.一般来说，细胞间距离越小，耦合率越大，但一些距离相近的细胞对的耦合率差异很大.细胞间的耦合也被注入一个外部水平细胞的Procion Yellow扩散到一个或两个相邻的外部水平细胞的发现所证明。不扩散到内部水平细胞或双极细胞.据报道，在相邻的外部水平细胞之间存在紧密的膜贴壁，但在这些细胞与双极细胞或内部水平细胞之间没有。在本研究中观察到的电连接的分布与这些解剖学发现一致。
1. In dogfish, studies were made of electrical connexions between pairs of cells giving S‐potentials. After recording, the types and locations of the two cells were determined morphologically by electrophoretically injecting two different fluorescent dyes.2. Hyperpolarizing S‐potentials were observed in external and internal horizontal cells; depolarizing responses were seen in bipolar cells. All pairs of external horizontal cells examined were electrically coupled, i.e. intra‐cellular polarization of one horizontal cell gave rise to polarization in its neighbour. Coupling was detected for cells separated by five other cells. The electrical coupling was non‐rectifying. No coupling was found between external and internal horizontal cells or between external horizontal cells and bipolar cells.3. Generally the smaller the distance between cells, the larger the coupling ratio, but some pairs of cells separated by similar distances showed widely different coupling ratios.4. The coupling between cells was also demonstrated by the finding that Procion Yellow injected into one external horizontal cell diffused into one or two neighbouring external horizontal cells. It did not diffuse into internal horizontal cells or bipolar cells.5. Close membrane apposition has been reported between neighbouring external horizontal cells, but not between these and either bipolar or internal horizontal cells. The distribution of electrical connexions observed in the present study agrees with these anatomical findings.