Erratum to: The guanine nucleotide exchange factor Vav3 regulates differentiation of progenitor cells in the developing mouse retina
Erratum to: The guanine nucleotide exchange factor Vav3 regulates differentiation of progenitor cells in the developing mouse retina
复制标题
勘误表：鸟嘌呤核苷酸交换因子 Vav3 调节小鼠视网膜发育中祖细胞的分化
DOI：
10.1007/s00441-015-2160-5
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发表时间：
2015
影响因子：
3.6
通讯作者：
Faissner A
中科院分区：
文献类型：
--
作者：
Luft V;Reinhard J;Shibuya M;Fischer KD;Faissner A
哺乳动物视网膜中的七种主要细胞类型来自多能视网膜祖细胞，这是一个受内在和外在信号严格调控的过程。然而，控制视网膜祖细胞增殖、分化和细胞命运决定的分子机制尚未完全了解。在这里，我们报告说，鸟嘌呤核苷酸交换因子Vav 3，调节Rho-GTPases，参与视网膜发育。我们证明，Vav 3在胚胎期的小鼠视网膜中表达。为了研究Vav 3在发育中的视网膜中的作用，我们产生了Vav 3缺陷小鼠。Vav 3的缺失导致在早期和晚期胚胎发育期间视网膜神经节细胞和视锥光感受器的加速分化。我们提供的证据表明，更多的视网膜祖细胞表达晚祖细胞标志物Sox 9在Vav 3缺陷小鼠比野生型。这种过早的分化在出生后得到补偿，晚出生的细胞类型如双极细胞和Müller胶质细胞显示正常数量。总之，我们的数据表明，Vav 3是视网膜祖细胞分化的调节因子，从而突出了鸟嘌呤核苷酸交换因子在视网膜发生中的新作用。
The seven main cell types in the mammalian retina arise from multipotent retinal progenitor cells, a process that is tightly regulated by intrinsic and extrinsic signals. However, the molecular mechanisms that control proliferation, differentiation and cell-fate decisions of retinal progenitor cells are not fully understood yet. Here, we report that the guanine nucleotide exchange factor Vav3, a regulator of Rho-GTPases, is involved in retinal development. We demonstrate that Vav3 is expressed in the mouse retina during the embryonic period. In order to study the role of Vav3 in the developing retina, we generate Vav3-deficient mice. The loss of Vav3 results in an accelerated differentiation of retinal ganglion cells and cone photoreceptors during early and late embryonic development. We provide evidence that more retinal progenitor cells express the late progenitor marker Sox9 in Vav3-deficient mice than in wild-types. This premature differentiation is compensated during the postnatal period and late-born cell types such as bipolar cells and Müller glia display normal numbers. Taken together, our data imply that Vav3 is a regulator of retinal progenitor cell differentiation, thus highlighting a novel role for guanine nucleotide exchange factors in retinogenesis.