Human RNase III is a 160-kDa protein involved in preribosomal RNA processing
Human RNase III is a 160-kDa protein involved in preribosomal RNA processing
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DOI：
10.1074/jbc.m005494200
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发表时间：
2000-11-24
影响因子：
4.8
通讯作者：
Crooke, ST
中科院分区：
文献类型：
--
作者：
Wu, HJ;Xu, H;Crooke, ST
人RNase III基因编码具有多个结构域的160 kDa的蛋白质，所述多个结构域为富含脯氨酸的结构域、富含丝氨酸和丝氨酸的结构域以及RNase III结构域。表达的纯化的RNase III结构域切割双链RNA，而不切割单链RNA。该基因在人体组织和细胞系中广泛表达，蛋白质定位于细胞核中，蛋白质的转录和翻译水平在细胞周期的不同阶段不会改变。然而，在细胞周期的S期期间，核中的蛋白质的显著部分易位到核仁。通过RNA酶III表达的反义抑制显示，这种人RNA酶III参与前体rRNA的加工，但可能在不同于酵母RNA酶III所描述的位点处切割。人RNase III表达的抑制导致细胞死亡，表明人RNase III在细胞中的重要作用。本研究所采用的反义抑制技术为新发现的人类基因的功能分析提供了一种有效的方法。
A human RNase III gene encodes a protein of 160 kDa with multiple domains, a proline-rich, a serine- and arginine-rich, and an RNase III domain. The expressed purified RNase III domain cleaves double-strand RNA and does not cleave single-strand RNA. The gene is ubiquitously expressed in human tissues and cell lines, and the protein is localized in the nucleus of the cell, The levels of transcription and translation of the protein do not change during different phases of the cell cycle. However, a significant fraction of the protein in the nucleus is translocated to the nucleolus during the S phase of the cell cycle, That this human RNase III is involved in processing of pre-rRNA, but might cleave at sites different from those described for yeast RNase III, is shown by antisense inhibition of RNase III expression. Inhibition of human RNase III expression causes cell death, suggesting an essential role for human RNase III in the cell. The antisense inhibition technique used in this study provides an effective method for functional analysis of newly identified human genes.