Intracellular localization of the severe acute respiratory syndrome coronavirus nucleocapsid protein: Absence of nucleolar accumulation during infection and after expression as a recombinant protein in Vero cells
Intracellular localization of the severe acute respiratory syndrome coronavirus nucleocapsid protein: Absence of nucleolar accumulation during infection and after expression as a recombinant protein in Vero cells
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DOI：
10.1128/jvi.79.17.11507-11512.2005
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发表时间：
2005-09-01
影响因子：
5.4
通讯作者：
Burton, MD
中科院分区：
文献类型：
--
作者：
Rowland, RRR;Chauhan, V;Burton, MD
在感染期间和用N基因转染细胞后，巢病毒目内的几个成员的核衣壳（N）蛋白定位于核仁。然而，在严重急性呼吸道综合征冠状病毒（SARS-CoV）感染或SARS-CoV N基因转染的Vero细胞中，N蛋白定位的共聚焦显微镜未能显示N在核质或核仁中的存在。氨基酸369至389，其中含有推定的核定位信号（NLS）和核仁定位信号基序，未能恢复核定位的NLS-减去突变Rev蛋白。这些数据表明，核定位是不是所有的巢状病毒之间的保守属性。
The nucleocapsid (N) protein of several members within the order Nidovirales localizes to the nucleolus during infection and after transfection of cells with N genes. However, confocal microscopy of N protein localization in Vero cells infected with the severe acute respiratory syndrome coronavirus (SARS-CoV) or transfected with the SARS-CoV N gene failed to show the presence of N in the nucleoplasm or nucleolus. Amino acids 369 to 389, which contain putative nuclear localization signal (NLS) and nucleolar localization signal motifs, failed to restore nuclear localization to an NLS-minus mutant Rev protein. These data indicate that nuclear localization is not a conserved property among all nidoviruses.