Differential expression of cell surface heparan sulfate proteoglycans in human mammary epithelial cells and lung fibroblasts.
Differential expression of cell surface heparan sulfate proteoglycans in human mammary epithelial cells and lung fibroblasts.
复制标题
人乳腺上皮细胞和肺成纤维细胞表面硫酸乙酰肝素蛋白聚糖的差异表达。
DOI：
10.1016/s0021-9258(18)48404-9
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Guido David
中科院分区：
文献类型：
--
作者：
V. Lories;J. Cassiman;H. V. D. Berghe;Guido David
用肝素酶和软骨素酶ABC处理来自人肺成纤维细胞和乳腺上皮细胞的脂质体可嵌入的硫酸乙酰肝素蛋白聚糖揭示了两种细胞类型中不同的核心蛋白模式。肺成纤维细胞表达具有约35、48/90（纤维聚糖）、64（磷脂酰肌醇蛋白聚糖）和125 kDa的核心蛋白的硫酸乙酰肝素蛋白聚糖以及携带硫酸乙酰肝素和硫酸软骨素链的杂交蛋白聚糖的痕量。相比之下，乳腺上皮细胞表达了大量的杂合蛋白聚糖和硫酸乙酰肝素蛋白聚糖，其核心蛋白约为35和64 kDa，但纤维聚糖和125 kDa的核心在这些细胞中检测不到。磷脂酰肌醇特异性磷脂酶C和单克隆抗体（mAb）S1确定的64 kDa的核心蛋白磷脂酰肌醇蛋白聚糖，而mAb 2 E9，也与小鼠乳腺上皮细胞的蛋白聚糖反应，暂时确定的杂合蛋白聚糖多配体聚糖。通过扩增成纤维细胞mRNA提取物中的多配体蛋白聚糖cDNA序列并证明编码的重组核心蛋白与mAb 2 E9的交叉反应性，证实了多配体蛋白聚糖在肺成纤维细胞中的表达。北方印迹未能检测到纤维聚糖在乳腺上皮细胞和其他几个上皮细胞系测试的消息，而确认的磷脂酰肌醇蛋白聚糖和syndecan在这些细胞中的表达。融合的成纤维细胞比指数生长的成纤维细胞表达更高水平的多配体蛋白聚糖mRNA，但这些水平仍然低于在上皮细胞中观察到的。这些数据正式鉴定人肺成纤维细胞的细胞表面蛋白聚糖之一为多配体聚糖，并表明细胞表面蛋白聚糖的表达在不同细胞类型和不同培养条件下变化。
Treating the liposome-intercalatable heparan sulfate proteoglycans from human lung fibroblasts and mammary epithelial cells with heparitinase and chondroitinase ABC revealed different core protein patterns in the two cell types. Lung fibroblasts expressed heparan sulfate proteoglycans with core proteins of approximately 35, 48/90 (fibroglycan), 64 (glypican), and 125 kDa and traces of a hybrid proteoglycan which carried both heparan sulfate and chondroitin sulfate chains. The mammary epithelial cells, in contrast, expressed large amounts of a hybrid proteoglycan and heparan sulfate proteoglycans with core proteins of approximately 35 and 64 kDa, but the fibroglycan and 125-kDa cores were not detectable in these cells. Phosphatidylinositol-specific phospholipase C and monoclonal antibody (mAb) S1 identified the 64-kDa core proteins as glypican, whereas mAb 2E9, which also reacted with proteoglycan from mouse mammary epithelial cells, tentatively identified the hybrid proteoglycans as syndecan. The expression of syndecan in lung fibroblasts was confirmed by amplifying syndecan cDNA sequences from fibroblastic mRNA extracts and demonstrating the cross-reactivity of the encoded recombinant core protein with mAb 2E9. Northern blots failed to detect a message for fibroglycan in the mammary epithelial cells and in several other epithelial cell lines tested, while confirming the expression of both glypican and syndecan in these cells. Confluent fibroblasts expressed higher levels of syndecan mRNA than exponentially growing fibroblasts, but these levels remained lower than observed in epithelial cells. These data formally identify one of the cell surface proteoglycans of human lung fibroblasts as syndecan and indicate that the expression of the cell surface proteoglycans varies in different cell types and under different culture conditions.