Expression of cell adhesion molecules in primary renal disease and renal allograft rejection
Expression of cell adhesion molecules in primary renal disease and renal allograft rejection
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DOI：
10.1093/ndt/12.6.1122
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发表时间：
1997-06-01
影响因子：
6.1
通讯作者：
Sterzel, RB
中科院分区：
文献类型：
--
作者：
Hauser, IA;Riess, R;Sterzel, RB
背景资料。体外研究表明，炎症介质如细胞因子TNF-α和IL-1可上调或诱导内皮细胞和上皮细胞表面细胞黏附分子的表达。本研究观察了66例原发性肾病患者和42例肾移植受者肾活检组织中细胞黏附分子ICAM-1、VCAM-1、E-选择素和PECAM-1的表达。冰冻切片用抗PECAM-1、ICAM-1、VCAM-1、E-选择素和MHC-II类分子的抗体(APAAP法)免疫组织化学方法检测细胞黏附分子的表达。PECAM-1和ICBM-1在肾血管内皮细胞表达，在内皮细胞破坏的闭塞肾小球中消失，在急性排斥反应和炎症性原发肾脏疾病中，ICAM-1在肾内皮细胞表达上调，而PECAM-1不表达。ICAM-1和VCAM-1在肾小管的从头表达与肾实质严重的结构损伤有关，包括间质纤维化、血管和/或肾小球VCAM-1和E-选择素。在严重的同种异体移植急性排斥反应中表达明显，也反映了伴或不伴自身免疫性疾病的原发肾脏疾病的炎症反应强度。VCAM-1和E-选择素在肾血管和/或肾小球中的从头表达和ICAM-1的过度表达是同种异体肾移植受者和原发肾脏疾病患者活检组织中急性和严重炎症过程的标志。
Background. In vitro studies have demonstrated that inflammatory mediators such as the cytokines TNF alpha and IL-1 upregulate or induce de novo expression of cell adhesion molecules on endothelial and epithelial cells, in the present study the expression of the cell adhesion molecules ICAM-1, VCAM-1, E-selectin and PECAM-1 was investigated in renal biopsies from patients with primary renal diseases (n = 66) and from renal allograft recipients (n = 42),Methods. Expression of the cell adhesion molecules was determined by immunohistochemistry of frozen sections using monoclonal antibodies directed against PECAM-1, ICAM-1, VCAM-1, E-selectin and MHC class II molecules (APAAP method).Results and Conclusions. PECAM-1 and ICBM-1 were expressed in the renal vasculature and disappeared in obliterated glomeruli with endothelial cell destruction, ICAM-1 but not PECAM-1 was upregulated in renal endothelia in acute allograft rejection and inflammatory primary renal diseases. Tubular de novo expression of ICAM-1 and VCAM-1 correlated with severe structural damage of the renal parenchyma including interstitial fibrosis, Vascular and/or glomerular VCAM-1 and E-selectin. expression was pronounced in severe acute allograft rejection and also reflected the intensity of inflammatory reactions in primary renal diseases with or without autoimmune disorders. De novo expression of VCAM-1 and E-selectin in renal vessels and/or glomeruli and overexpression of ICAM-1 are markers of acute and severe inflammatory processes in biopsies from allograft recipients and patients with primary renal diseases.