Complex regulatory network encompassing the Csr, c-di-GMP and motility systems of Salmonella Typhimurium.
Complex regulatory network encompassing the Csr, c-di-GMP and motility systems of Salmonella Typhimurium.
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DOI：
10.1111/j.1462-2920.2009.02097.x
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发表时间：
2010-02
影响因子：
5.1
通讯作者：
Melefors O
中科院分区：
文献类型：
--
作者：
Jonas K;Edwards AN;Ahmad I;Romeo T;Römling U;Melefors O
细菌的生存依赖于其根据环境条件的变化在固着和运动生活方式之间转换的能力。在许多物种中，这种转换受一种信号分子——（3′ - 5′）-环二鸟苷酸（c - di - GMP）的调控，它由含有GGDEF和/或EAL结构域的蛋白质代谢。鼠伤寒沙门氏菌含有20种此类蛋白质。在此，我们表明RNA结合蛋白CsrA调控8个编码GGDEF、GGDEF - EAL和EAL结构域蛋白质的基因的表达。CsrA直接结合到其中5个基因的mRNA前导序列上，这表明它可能在转录后调控这些基因。c - di - GMP特异性磷酸二酯酶STM3611，它相互控制鞭毛功能和生物膜基质成分的产生，受CsrA与mRNA的结合调控，但也通过FlhDC/FliA鞭毛级联和STM1344受到CsrA的间接调控。STM1344是一种非常规的（c - di - GMP无活性）EAL结构域蛋白质，最近被鉴定为鞭毛基因表达的负调控因子。在此，我们证明CsrA直接下调STM1344的表达，而STM1344又通过fliA调控STM3611，从而相互控制运动性和生物膜因子。总之，我们的数据揭示了对几个编码GGDEF/EAL结构域蛋白质的基因的协同和复杂调控使得CsrA能够在多个层面上控制鼠伤寒沙门氏菌的运动 - 固着转换。
Bacterial survival depends on the ability to switch between sessile and motile life styles in response to changing environmental conditions. In many species, this switch is governed by (3′-5′)-cyclic-diguanosine monophosphate (c-di-GMP) a signalling molecule, which is metabolized by proteins containing GGDEF and/or EAL domains. Salmonella Typhimurium contains 20 such proteins. Here, we show that the RNA-binding protein CsrA regulates the expression of eight genes encoding GGDEF, GGDEF-EAL and EAL domain proteins. CsrA bound directly to the mRNA leaders of five of these genes, suggesting that it may regulate these genes post-transcriptionally. The c-di-GMP specific phosphodiesterase STM3611, which reciprocally controls flagella function and production of biofilm matrix components, was regulated by CsrA binding to the mRNA, but was also indirectly regulated by CsrA through the FlhDC/FliA flagella cascade and STM1344. STM1344 is an unconventional (c-di-GMP-inactive) EAL domain protein, recently identified as a negative regulator of flagella gene expression. Here, we demonstrate that CsrA directly downregulates expression of STM1344, which in turn regulates STM3611 through fliA and thus reciprocally controls motility and biofilm factors. Altogether, our data reveal that the concerted and complex regulation of several genes encoding GGDEF/EAL domain proteins allows CsrA to control the motility-sessility switch in S. Typhimurium at multiple levels.
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影响因子：
3.6
作者：
Hisert, KB;MacCoss, M;Nathan, C
通讯作者：
Nathan, C
影响因子：
5.6
作者：
Ko, M;Park, C
通讯作者：
Park, C
影响因子：
4.8
作者：
Claret, Laurent;Miquel, Sylvie;Darfeuille-Michaud, Arlette
通讯作者：
Darfeuille-Michaud, Arlette
DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Datsenko, KA;Wanner, BL
通讯作者：
Wanner, BL
影响因子：
3.2
作者：
Dubey, AK;Baker, CS;Babitzke, P
通讯作者：
Babitzke, P