A bacterial spore test piece for the control of ethylene oxide sterilization.
A bacterial spore test piece for the control of ethylene oxide sterilization.
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一种用于控制环氧乙烷灭菌的细菌芽孢试片。
DOI：
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发表时间：
1965
期刊：
影响因子：
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通讯作者：
C. Whitehouse
中科院分区：
文献类型：
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作者：
M. M. Beeby;C. Whitehouse
检查了带有不同数量枯草芽孢杆菌Camp Detrick菌株孢子的铝箔条（从水、90%（v/v）甲醇、5%和20%（v/v）血清、营养肉汤和等渗盐水中干燥），以确定其是否可用作环氧乙烷灭菌控制的试验件。发现这些孢子的甲醇悬浮液在储存时是稳定的，并且携带从甲醇干燥的孢子的箔是测试的那些中最可再现和最稳定的。通过改变每个铝箔上的孢子数量和每次试验使用的铝箔数量，可以控制试样系统的敏感性。
Aluminium foil strips carrying varying numbers of spores of the Camp Detrick strain of Bacillus subtilis dried from water, 90% (v/v) methanol, 5 and 20% (v/v) serum, nutrient broth and isotonic saline were examined for their possible use as test pieces for the control of sterilization by ethylene oxide. Methanolic suspensions of these spores were found to be stable on storage, and foils carrying spores dried from methanol were the most reproducible and stable of those tested. The susceptibility of the test piece system could be controlled by varying the numbers of spores on each foil and the numbers of foils used per test.