An Fgf8 mutant allelic series generated by Cre- and Flp-mediated recombination
An Fgf8 mutant allelic series generated by Cre- and Flp-mediated recombination
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DOI：
10.1038/ng0298-136
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发表时间：
1998-02-01
期刊：
影响因子：
30.8
通讯作者：
Martin, GR
中科院分区：
文献类型：
--
作者：
Meyers, EN;Lewandoski, M;Martin, GR
我们描述了一种策略，用于产生一个等位基因系列的突变，在一个给定的基因座，只需要生产一个靶向小鼠线。我们生产的“等位基因”小鼠系携带Fgf8的亚纯型等位基因，其可以通过与cre转基因动物交配而转化为无效等位基因。通过使等位基因小鼠与flp转基因动物交配，也可以将亚纯型等位基因恢复为野生型，从而产生适合于Cre诱导的组织特异性敲除实验的小鼠品系。对携带这些等位基因的不同组合的胚胎的分析揭示了在原肠胚形成期间以及心脏、颅面、前脑、中脑和小脑发育期间对Fgf8基因功能的要求。
We describe a strategy for generating an allelic series of mutations at a given locus that requires the production of only one targetted mouse line. The 'allelogenic' mouse line we produced carries a hypomorphic allele of Fgf8, which can be converted to a null allele by mating to cre transgenic animals. The hypomorphic allele can also be reverted to wild-type by mating the allelogenic mice to flp transgenic animals, thereby generating a mouse line suitable for Cre-induced tissue-specific knockout experiments. Analysis of embryos carrying different combinations of these alleles revealed requirements for Fgf8 gene function during gastrulation, as well as cardiac, craniofacial, forebrain, midbrain and cerebellar development.