The anatase phase of nanotopography titania plays an important role on osteoblast cell morphology and proliferation
The anatase phase of nanotopography titania plays an important role on osteoblast cell morphology and proliferation
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纳米形貌二氧化钛的锐钛矿相对成骨细胞形态和增殖起着重要作用
DOI：
10.1007/s10856-008-3505-3
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发表时间：
2008-11-01
影响因子：
3.7
通讯作者：
Chen, Wantao
中科院分区：
文献类型：
--
作者：
He, Jie;Zhou, Wei;Chen, Wantao
生物材料的表面特性对细胞的粘附、迁移、增殖和分化等形态和行为起着至关重要的作用。采用直流反应磁控溅射法制备了粗糙度相近的金红石型、锐钛型和非晶型三种不同晶相的二氧化钛薄膜。各膜的表面粗糙度约为8 ~ 10 nm。用原代大鼠成骨细胞观察其形态变化，并评价细胞在膜表面的行为。孵育36 h和72 h后，锐钛矿膜上的成骨细胞数量明显高于金红石膜和无定形膜。更重要的是，在锐钛矿膜上培养的成骨细胞在7天和14天后，碱性磷酸酶的合成显著高于金红石膜和无定形膜。在锐钛矿相膜上生长的细胞扩展面积最大；具有规则方向的细胞内肌动蛋白丝轮廓清晰，充分展开。结果表明，纳米形貌的锐钛矿相对细胞的粘附、扩散、增殖和分化具有最佳的生物学效应。该生物材料薄膜具有很强的成骨细胞增殖治疗前景，在骨科和牙科种植等方面具有潜在的应用前景。
The surface properties of biomaterials play a vital role in cell morphology and behaviors such as cell adhesion, migration, proliferation and differentiation. Three different crystal phases of titania film (rutile, anatase and amorphous titania) with similar roughness were successfully synthesized by DC reactive magnetron sputtering. The surface roughness of each film was about 8–10 nm. Primary rat osteoblasts were used to observe changes in morphology and to evaluate cell behavior at the film surface. The number of the osteoblasts on anatase film was significantly higher than rutile and amorphous films after 36 and 72 h incubation. More importantly, synthesis of alkaline phosphatase was significantly greater by osteoblasts cultured on anatase film than on rutile and amorphous films after 7 and 14 days. In addition, the cells grown on the anatase phase film had the largest spreading area; the actin filaments in cells with regular directions were well defined and fully spreaded. The results indicate that the anatase phase of titania with nanoscale topography yield the best biological effects for cell adhesion, spreading, proliferation and differentiation. There are strong therapeutic prospects for this biomaterial film for osteoblast proliferation, with possible applications for orthopedic and dental implant.