A robust circadian rhythm of metabolites in Arabidopsis thaliana mutants with enhanced growth characteristics
A robust circadian rhythm of metabolites in Arabidopsis thaliana mutants with enhanced growth characteristics
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DOI：
10.1371/journal.pone.0218219
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发表时间：
2019-06-25
期刊：
影响因子：
3.7
通讯作者：
Alia, A.
中科院分区：
文献类型：
--
作者：
Augustijn, Dieuwertje;de Groot, Huub J. M.;Alia, A.
气候变化和不断增长的粮食需求需要能够产生更多生物量的智能作物。最近，通过基因表达的全基因组重编程，获得了两个具有增强生长特征的拟南芥突变体VP16-02-003和VP16-05-014，并鉴定了这些增强生长表型的新生物标志物。由于昼夜节律周期强烈影响代谢和生理过程，并对促进生长的光合机制施加控制，因此在本研究中，我们研究了昼夜节律时钟对大莲座表面积表型的18个关键生物标志物代谢节律的影响。利用高分辨率魔角旋转（HR-MAS）核磁共振研究了整个昼夜周期中七个不同时间点的完整叶片的代谢谱。结果表明，野生型col0和VP16-02-003以及VP16-05-014突变体的生物标志物代谢物的昼夜节律非常强劲，与col0相比，这两种突变体在整个昼夜周期中的代谢物水平差异很大。我们的分析表明，稳健性是通过生物钟和主要代谢过程之间的功能独立性来实现的。
Climate change and the rising food demand provide a need for smart crops that yield more biomass. Recently, two Arabidopsis thaliana mutants with enhanced growth characteristics, VP16-02-003 and the VP16-05-014, were obtained by genome-wide reprogramming of gene expression, which led to the identification of novel biomarkers of these enhanced growth phenotypes. Since the circadian cycle strongly influences metabolic and physiological processes and exerts control over the photosynthetic machinery responsible for enhanced growth, in this study, we investigate the influences of the circadian clock on the metabolic rhythm of eighteen key biomarkers for the larger rosette surface area phenotype. The metabolic profile was studied in intact leaves at seven different time points throughout the circadian cycle using high-resolution magic angle spinning (HR-MAS) NMR. The results show that the circadian rhythm of biomarker metabolites are remarkably robust across wild-type Col-0 and VP16-02-003 and the VP16-05-014 mutants, with widely different metabolite levels of both mutants compared to Col-0 throughout the circadian cycle. Our analysis reveals that robustness is achieved through functional independence between the circadian clock and primary metabolic processes.