Luteinizing hormone reduces the activity of the NPR2 guanylyl cyclase in mouse ovarian follicles, contributing to the cyclic GMP decrease that promotes resumption of meiosis in oocytes.
Luteinizing hormone reduces the activity of the NPR2 guanylyl cyclase in mouse ovarian follicles, contributing to the cyclic GMP decrease that promotes resumption of meiosis in oocytes.
复制标题
DOI：
10.1016/j.ydbio.2012.04.019
复制
发表时间：
2012-06-15
影响因子：
2.7
通讯作者：
Jaffe LA
中科院分区：
文献类型：
--
作者：
Robinson JW;Zhang M;Shuhaibar LC;Norris RP;Geerts A;Wunder F;Eppig JJ;Potter LR;Jaffe LA
在小鼠排卵前卵泡中，卵母细胞减数分裂前期停滞是由周围颗粒细胞的环 GMP 通过间隙连接扩散到卵母细胞中维持的。 cGMP 在颗粒细胞中由跨膜鸟苷酸环化酶利尿钠肽受体 2 (NPR2) 响应激动剂 C 型利钠肽 (CNP) 合成。为了响应黄体生成素 (LH)，颗粒细胞中的 cGMP 降低，结果是卵母细胞 cGMP 降低并且减数分裂恢复。在这里，我们报告，在 20 分钟内，LH 处理导致 NPR2 鸟苷酸环化酶活性降低，这是在最大激活浓度的 CNP 存在下测定的。这是通过不减少 NPR2 蛋白量的过程发生的。我们还表明，通过一个较慢的过程（在 2 小时首次检测到），LH 减少了可与受体结合的 CNP 的量。这两种 LH 作用都有助于降低卵泡中的 cGMP，从而发出卵母细胞减数分裂恢复的信号。
In preovulatory ovarian follicles of mice, meiotic prophase arrest in the oocyte is maintained by cyclic GMP from the surrounding granulosa cells that diffuses into the oocyte through gap junctions. The cGMP is synthesized in the granulosa cells by the transmembrane guanylyl cyclase natriuretic peptide receptor 2 (NPR2) in response to the agonist C-type natriuretic peptide (CNP). In response to luteinizing hormone (LH), cGMP in the granulosa cells decreases, and as a consequence, oocyte cGMP decreases and meiosis resumes. Here we report that within 20 minutes, LH treatment results in decreased guanylyl cyclase activity of NPR2, as determined in the presence of a maximally activating concentration of CNP. This occurs by a process that does not reduce the amount of NPR2 protein. We also show that by a slower process, first detected at 2 hours, LH decreases the amount of CNP available to bind to the receptor. Both of these LH actions contribute to decreasing cGMP in the follicle, thus signaling meiotic resumption in the oocyte.
登录
查看更多内容
影响因子：
4.6
作者：
Norris, Rachael P.;Freudzon, Marina;Mehlmann, Lisa M.;Cowan, Ann E.;Simon, Alexander M.;Paul, David L.;Lampe, Paul D.;Jaffe, Laurinda A.
通讯作者：
Jaffe, Laurinda A.
影响因子：
4.8
作者：
Dickey, Deborah M.;Burnett, John C., Jr.;Potter, Lincoln R.
通讯作者：
Potter, Lincoln R.
影响因子：
5.8
作者：
Abbey-Hosch, SE;Smirnov, D;Potter, LR
通讯作者：
Potter, LR
影响因子：
4.6
作者：
Norris, Rachael P.;Ratzan, William J.;Jaffe, Laurinda A.
通讯作者：
Jaffe, Laurinda A.
影响因子：
4.8
作者：
Chattopadhyay, A;Vecchi, M;Carpenter, G
通讯作者：
Carpenter, G