Age-associated analysis of oxidative stress parameters in human plasma and erythrocytes
Age-associated analysis of oxidative stress parameters in human plasma and erythrocytes
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DOI：
10.1080/10715760600592962
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发表时间：
2006-05-01
影响因子：
3.3
通讯作者：
Grune, T
中科院分区：
文献类型：
--
作者：
Gil, L;Siems, W;Grune, T
氧化损伤在大分子中的积累被认为是细胞损伤和病理的原因。很少有健康人的氧化应激参数与个体年龄相关的报道。本研究的目的是检查健康个体的血浆和红细胞中的氧化还原状态，并确定这些参数与衰老过程之间的相关性。使用以下参数：194名年龄从18岁至84岁的健康女性和男性的血液和血浆样品中的丙二醛（MDA）、蛋白质羰基（PCO）、4-羟基-2，3-反式-壬烯醛（HNE）、还原型谷胱甘肽（GSH）、谷胱甘肽二硫化物（GSSG）和尿酸（UA）。结果表明，血浆中氧化剂和抗氧化剂系统的平衡在老化过程中有利于加速氧化。MDA、HNE、GSSG随年龄增长而增加，红细胞GSH随年龄增长而略有下降。由于UA的含量更多地取决于代谢和营养影响，而不是促氧化剂和抗氧化剂之间的平衡，因此没有观察到显着的年龄相关变化。对于HNE的血浆浓度，首次给出了健康人的年龄依赖性参考值。
Oxidative damage accumulation in macromolecules has been considered as a cause of cellular damage and pathology. Rarely, the oxidative stress parameters in healthy humans related to the individual age have been reported. The purpose of this study was to examine the redox status in plasma and erythrocytes of healthy individuals and determine correlations between these parameters and the aging process. The following parameters were used: malondialdehyde (MDA), protein carbonyls (PCO), 4-hydroxy-2,3- trans -nonenal (HNE), reduced glutathione (GSH), glutathione disulfide (GSSG) and uric acid (UA) in blood and plasma samples of 194 healthy women and men of ages ranging from 18 to 84 years. The results indicate that the balance of oxidant and antioxidant systems in plasma shifts in favor of accelerated oxidation during ageing. That is demonstrated by increases of MDA, HNE, GSSG and by the slight decrease of erythrocytic GSH with age. As the content of UA is more determined by metabolic and nutritional influences than by the balance between prooxidants and antioxidants there was no significant age-related change observed. For plasma concentrations of HNE the first time age-dependent reference values for healthy humans are presented.